求单词slide的翻译.(有背景文献)

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背景文献如下:1 z0 _- c- G6 n
For osteogenic differentiation, fourth passage MSC were
8 @. B- b) A4 d+ Q0 fplated at 5×103 cells/cm2 in a 9.6-cm2 slideflask (Nunc,4 ], O* k# C2 ~6 f' ^; A
Denmark), grown up to 80% confluence, and incubated in/ }' x' b7 x( J  H( n
osteogenic medium (NH Osteodiff Medium, Miltenyi
3 G! z6 \9 R3 U& `  n; t; [Biotec, Germany). The medium was replaced every 3–4 days. After 10 days, cultures were washed with PBS and6 z7 B; R/ K8 N' U* s2 F  ^9 N* O( ~
fixed in a solution of ice-cold 70% ethanol for 10 min. For
* c8 G  T* v* G1 a, Y7 G9 qalkaline phosphatase staining, slides were fixed in 100%' r% R( m3 k7 ~$ O, S3 J
methanol for 5 min, washed with PBS, and then incubated0 y* `: N: M5 k( V# O
with 5-bromo-4-chloro3-indolyl phosphate/nitro blue tetrazolium
5 j  \% a) I5 J6 ~2 Z(BCIP-NBT, Sigma-Aldrich) for 15–30 min, strictly
6 o7 {4 W* l# P7 n: v1 o0 Jfollowing manufacturer’s instructions