流式分析造血干细胞

luozhen1018

求助：小鼠骨髓中造血干细胞的比例及其各系细胞所占比例的参考范围？' c( I3 x3 `. ^) L5 P
谢谢！

iseeyou1210

it depends on the celluar suface marker you used.

luozhen1018

干细胞标志是lin- c-kit+ Scal+
) f7 ^9 t! M% X) o红系：Ter119
- u4 B/ X5 N9 @5 ]B淋巴细胞：B220+ S9 L3 t9 ^. d% t& C
T淋巴细胞：CD4、CD8
  K( o: }. O9 l* r7 U: f髓系：CD11b、Gr-1

luozhen1018

干细胞标志是lin- c-kit+ Scal+
! _( u" t/ {8 L7 {5 p/ w8 @1 T- z2 n红系：Ter119
: ^3 k0 ~" ]0 J: a: TB淋巴细胞：B2208 S3 @. y7 T5 }. L8 @) T/ C
T淋巴细胞：CD4、CD83 W5 Z2 g& B) p& p8 x6 ?
髓系：CD11b、Gr-1

future32

xiexie

louise33

It really depends on which stage of cells that you are going to analyse. ) F. v* q: F! M$ a, S" _
Granulocytes: Gr1+Mac1++ ~8 W: v$ R" F
Myeloid cells: Mac 1+ Gr 1-
( R4 E- p! y' k6 D" w: |4 rMyeloid progenitors: Mac1+Kit+7 O' ~- E& A. X' e, ?, d3 P
NK cells: NK1.1++ `! d8 H+ H* B# [" w2 ~  K" m
MPP: LSK CD150-5 ^3 g$ r( T0 I6 V- Q+ u* K
Long-term HSC: LSK CD34-7 y; T. ]4 D6 o8 `
LMPP: LSK CD34+FLT3+' j$ \7 q# t# U) ]+ [# M: d
ST-HSC: LSK CD34+  ?$ x6 @! A# p; r; c
, H1 p" n: o% }* m; K$ V# ^
for more, please refer to the attachment, you can gain more insight!
$ z' J6 ?4 H7 z% m) \! R5 J; Y: a3 j5 @% ?3 ?# |

montalexiong

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