流式分析造血干细胞

luozhen1018

求助：小鼠骨髓中造血干细胞的比例及其各系细胞所占比例的参考范围？
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iseeyou1210

it depends on the celluar suface marker you used.

luozhen1018

干细胞标志是lin- c-kit+ Scal+8 [: R1 w5 B* \" V
红系：Ter119
: X" {  o. F# G. ?- _6 @2 @B淋巴细胞：B220' H6 `1 d! T! T$ l0 `
T淋巴细胞：CD4、CD8
& N' O" j. A% J) {" B) H髓系：CD11b、Gr-1

luozhen1018

干细胞标志是lin- c-kit+ Scal+
) N$ b7 A4 C- L: \% I红系：Ter119
, {+ ^9 f0 G) P0 l0 E% |B淋巴细胞：B220
  {+ o# Y4 l3 [# GT淋巴细胞：CD4、CD8
9 G8 V1 Y( a# U, l: `髓系：CD11b、Gr-1

future32

xiexie

louise33

It really depends on which stage of cells that you are going to analyse. . U6 {3 e( I  ~1 r5 k
Granulocytes: Gr1+Mac1+3 x7 O' H" j% N, k+ k. P; `
Myeloid cells: Mac 1+ Gr 1-
  u4 P8 x4 a) F0 F- D# v) l' WMyeloid progenitors: Mac1+Kit+
1 ^$ Z7 I/ V# P: i& P4 zNK cells: NK1.1+4 R8 ^% m! [# F& F+ {9 F+ ~* d& e# ^
MPP: LSK CD150-- ?( w6 x" J# u8 [; E
Long-term HSC: LSK CD34-
9 y3 A4 g' ?) L% j, ?0 Y" YLMPP: LSK CD34+FLT3+
9 C3 v! y1 ]; \5 u4 |" tST-HSC: LSK CD34+2 }- j/ F( G. |# m$ |
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for more, please refer to the attachment, you can gain more insight!
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montalexiong

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