PDF电子书：Current_Protocols_in_Stem_Cell_Biology

guokeyi

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FOREWORD
& Y3 d( `+ O3 s9 K7 fStem cell biology is emerging as a field in biology with tremendous therapeutic potential.
) M/ I+ I/ J9 u# o- H" o" iMaking this potential a reality requires an international effort. The recognition! _; @0 }1 @0 t
that such a promising yet multifaceted discipline needs fostering led to the establishment
/ E# T' b+ g+ h* k+ t& p& l) Kof the International Society for Stem Cell Research (ISSCR). Central to the efforts of the9 V8 X2 r  M: V
ISSCR is the development of tools to ensure the success of stem cell researchers. What, w# v- ?" F+ q; i3 b
better way to do this than to collaborate with Current Protocols to develop this valuable) l& K, ^, n0 t4 z
compendium of protocols in stem cell biology?4 [+ o! |. i. r7 A) {8 l/ i6 ~$ O. {
Stem cell researchers have developed a number of breakthrough techniques, from the6 H6 Z! j; n% p
derivation and manipulation of pluripotent embryonic stem cells to purification and
# N' v* W& x6 n: v$ h+ |) w) z) Ttransplantation of tissue-restricted stem cells from adult organs. A number of laboratories
/ s) |4 P% ?# C2 P) {( p, Bhave become leaders in the field as a result of developing such techniques. The more
( s5 m* X( k$ d* Uefficient scientists are at implementing new and powerful methodologies in their own
* V- x. I  W! i* r; K6 Elaboratories, the faster stem cell biology will advance our understanding of normal# d  \" J7 \( k3 A+ W
development and lead to the development of therapies. Thus, the availability of quality
6 U' t9 D6 Q+ @' E" A3 I2 O, Fprotocols will have amajor impact on the success of the entire field. Current Protocols has5 i) Q8 p& D4 a) N8 z. V: {4 ~8 ~
long been the premier volume for proven in-depth protocols regarding many aspects of& `' V* Y* B( y4 Z3 s" D
biology, and this volume on stem cell biology will prove a valuable addition to researchers4 i; _' k' O0 T
worldwide./ [; u5 Y. ~" y! J' z3 R6 {
Experiments in stem cell biology must be interpreted with great caution as well as
2 u5 r1 S1 @& L! Vopenness to alternative explanations. For example, the recently discovered phenomenon
% P* h7 r8 J! H5 Yof cell fusion in vivo or the existence of tissue-restricted blood stem cells in peripheral
, P) M; q% `! F+ g# s* T6 U) l- M$ otissues were initially misinterpreted as evidence for stem cell trans-differentiation.
4 G; j  h) e: O# HIt is very important that this compendium of protocols highlight potential pitfalls as well% s5 Y0 V2 H- e# H$ }. T2 V
as maintain the opportunity for clarification and correction when the need arises. The7 f6 a5 @0 w' g# R* j. J# S
fact that these protocols will be provided online will help ensure that researchers always
/ ]9 E; h) P, F% E( S1 l% dhave the latest, most up-to-date protocols available to them.
& W% ^% m0 |5 @2 ?" s' FThe stem cell field is burgeoning, and, as I have seen within the ISSCR, there is a genuine# \- r, ^: b: T6 ]! f
push to share information and interact so that the field can move forward quickly. There
5 X' R& N& \2 h4 K: g/ _' kis a drive to develop not only excellent basic research skills but to bring the findings
# I' F4 H/ A8 nto clinical use so that patients can benefit. As a Hematology Attending Physician at" Y  t! j9 M2 B$ u* U0 U3 ^; x- t
Children’s HospitalBoston, I treat children who have pediatric blood diseases or leukemia
! ~* }8 \; }" J- Z% C: f. Tand am drawn by the need to translate our research findings into therapies to help treat a7 F" r4 S0 ~& W/ R/ `' z4 S
number of diseases. The promise is great, but we need to deliver, and I believe Current
6 O2 x( j( Q$ V% U. BProtocols in Stem Cell Biology will help tremendously.
9 h/ N9 N3 M/ X$ ^0 YLeonard I. Zon
9 P! T% T+ ~) `Current Protocols in Stem Cell Biology  b% _) P# L% A, O1 i8 E
Published online June 2007 in Wiley Interscience (www.interscience.wiley.com).
7 C$ f% v  i; j8 W' w+ ?DOI: 10.1002/9780470151808.scfores18 }. n0 h2 X+ y7 R
Copyright C
2007 John
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