PDF电子书：Current_Protocols_in_Stem_Cell_Biology

guokeyi

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" N9 p* ~2 H6 |7 W6 L1 \* l1 KFOREWORD
2 \# H/ r" u. d; c& `1 wStem cell biology is emerging as a field in biology with tremendous therapeutic potential.! H! R8 v; F. H& Y. X
Making this potential a reality requires an international effort. The recognition+ p0 Z8 S, y4 p7 D
that such a promising yet multifaceted discipline needs fostering led to the establishment
  [+ _" ]# q+ `1 g) n' gof the International Society for Stem Cell Research (ISSCR). Central to the efforts of the
6 B$ W& F+ S6 J* mISSCR is the development of tools to ensure the success of stem cell researchers. What/ l' [: K7 _0 B- R4 B4 g9 y
better way to do this than to collaborate with Current Protocols to develop this valuable
- @; h- [4 t3 e  Scompendium of protocols in stem cell biology?
- i- h9 b: j& ?6 E# HStem cell researchers have developed a number of breakthrough techniques, from the
7 d! i( x" ?; @! _8 |: Wderivation and manipulation of pluripotent embryonic stem cells to purification and
1 n2 p, F0 b8 J/ etransplantation of tissue-restricted stem cells from adult organs. A number of laboratories
2 S( r1 o% T) z9 G  Q, Nhave become leaders in the field as a result of developing such techniques. The more7 e( l8 _& H5 @7 e$ Q' n
efficient scientists are at implementing new and powerful methodologies in their own8 ?. `1 a: S  H  c9 c. V$ J
laboratories, the faster stem cell biology will advance our understanding of normal' j+ d% ]: [( v/ ~
development and lead to the development of therapies. Thus, the availability of quality) \' E* s2 P* s% z
protocols will have amajor impact on the success of the entire field. Current Protocols has
4 u+ ~% m. o2 G* `( r+ Q5 Y  M) Along been the premier volume for proven in-depth protocols regarding many aspects of; x& V/ Q+ G9 }, h5 d
biology, and this volume on stem cell biology will prove a valuable addition to researchers. N4 [1 `% R% h, s0 C  W. _
worldwide.
# @9 L; l( A9 e: E% h/ r) ^Experiments in stem cell biology must be interpreted with great caution as well as
1 o3 V/ g) L5 `% popenness to alternative explanations. For example, the recently discovered phenomenon+ P0 N' {3 z0 R$ f
of cell fusion in vivo or the existence of tissue-restricted blood stem cells in peripheral
4 I" \. W6 i$ S+ @3 @tissues were initially misinterpreted as evidence for stem cell trans-differentiation.
- i9 ~  m- o& a+ E" c/ H5 LIt is very important that this compendium of protocols highlight potential pitfalls as well) h$ ^. `) o' u, X1 W
as maintain the opportunity for clarification and correction when the need arises. The
' }; j) a. u. h5 o: Z+ x$ Ufact that these protocols will be provided online will help ensure that researchers always( B/ _* o4 ?' D( ~, M+ B3 U  l, I/ H
have the latest, most up-to-date protocols available to them.* v2 M9 G: g2 ^& b' M! h% y7 U6 g3 f
The stem cell field is burgeoning, and, as I have seen within the ISSCR, there is a genuine
+ R0 [% M7 C! |7 {push to share information and interact so that the field can move forward quickly. There8 p0 n0 s2 J# u/ f# L
is a drive to develop not only excellent basic research skills but to bring the findings5 Y& b7 \! w; i8 l; d% y% Z
to clinical use so that patients can benefit. As a Hematology Attending Physician at+ K1 e. a) J: t- n; R& X" N) t
Children’s HospitalBoston, I treat children who have pediatric blood diseases or leukemia( A) v7 K& {- z7 D. H, q4 z+ f
and am drawn by the need to translate our research findings into therapies to help treat a: @! c* F  f6 j; M0 n4 c) U
number of diseases. The promise is great, but we need to deliver, and I believe Current) u, u; f6 B  P2 w% P
Protocols in Stem Cell Biology will help tremendously.* P8 S) n$ G2 z! s! j* P
Leonard I. Zon
8 d5 W0 y% Y  SCurrent Protocols in Stem Cell Biology! s' Z# c3 p4 s) g+ m3 y) T2 S' T! P
Published online June 2007 in Wiley Interscience (www.interscience.wiley.com).- y9 a' m! ^" V% o' u5 t" D* p
DOI: 10.1002/9780470151808.scfores11 d5 ~6 ^9 [' A# T' d! M1 N( ~2 w
Copyright C
2007 John4 u: ^. e3 {2 P( v: k% x

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xiaodongqin

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Jonathan

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zpzp0312

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zisu_yu

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horst

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dragon513

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