PDF电子书：Current_Protocols_in_Stem_Cell_Biology

guokeyi

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FOREWORD5 ~8 a6 U/ p7 C
Stem cell biology is emerging as a field in biology with tremendous therapeutic potential.
9 h3 O" b! k% `% KMaking this potential a reality requires an international effort. The recognition3 \6 \! ]8 O: ^- w( X1 g- x
that such a promising yet multifaceted discipline needs fostering led to the establishment# T- O, m0 y* w' u. c
of the International Society for Stem Cell Research (ISSCR). Central to the efforts of the% z  `6 ]+ i; M- ^8 D4 T0 o. M
ISSCR is the development of tools to ensure the success of stem cell researchers. What  s5 g7 a  [8 x) g0 q7 g
better way to do this than to collaborate with Current Protocols to develop this valuable/ \9 b' m, L  P5 B
compendium of protocols in stem cell biology?
& J: E+ r" y. f6 vStem cell researchers have developed a number of breakthrough techniques, from the  g# j( t) f- |* T% x
derivation and manipulation of pluripotent embryonic stem cells to purification and4 [7 G! {! w& n9 g% X- k8 r2 p) |( _
transplantation of tissue-restricted stem cells from adult organs. A number of laboratories
' v2 Z: B1 q4 c. uhave become leaders in the field as a result of developing such techniques. The more
! ]: r. b) u9 M% P) G! t" Y( Iefficient scientists are at implementing new and powerful methodologies in their own
- p3 n3 N7 R9 nlaboratories, the faster stem cell biology will advance our understanding of normal
" f$ \7 ]! U9 sdevelopment and lead to the development of therapies. Thus, the availability of quality
1 A8 \8 L, x2 r' C6 }. c0 I% Pprotocols will have amajor impact on the success of the entire field. Current Protocols has
$ @$ Z# N. [, ]% _2 u6 nlong been the premier volume for proven in-depth protocols regarding many aspects of
4 f) ]: p3 A* f  {6 l$ Ubiology, and this volume on stem cell biology will prove a valuable addition to researchers
6 d0 I7 H2 }% y6 aworldwide.' X8 [9 E  g" m9 f2 K
Experiments in stem cell biology must be interpreted with great caution as well as
% D6 X! C9 b, I5 e7 Copenness to alternative explanations. For example, the recently discovered phenomenon
3 J: B5 I, d. q/ l$ Iof cell fusion in vivo or the existence of tissue-restricted blood stem cells in peripheral
3 \, b* g# W5 {# n* B' E, g9 Wtissues were initially misinterpreted as evidence for stem cell trans-differentiation.
5 T# M. ]6 q9 s/ F9 _) f8 YIt is very important that this compendium of protocols highlight potential pitfalls as well
2 L8 C! r8 F) b+ C3 G$ das maintain the opportunity for clarification and correction when the need arises. The5 J) C$ G) ]2 L6 z$ ~
fact that these protocols will be provided online will help ensure that researchers always
. E" b+ ^: P- u8 }0 ]have the latest, most up-to-date protocols available to them.
3 C/ n# M  m) O0 p' yThe stem cell field is burgeoning, and, as I have seen within the ISSCR, there is a genuine" h- k& s0 R) O2 S
push to share information and interact so that the field can move forward quickly. There' t: X2 q3 u  N4 o8 ]5 [
is a drive to develop not only excellent basic research skills but to bring the findings5 b. {8 N  k- Q
to clinical use so that patients can benefit. As a Hematology Attending Physician at% M' L$ l: F) d# s" @0 X; Y
Children’s HospitalBoston, I treat children who have pediatric blood diseases or leukemia2 m: k# U/ p9 v! l4 f1 Q6 y4 k
and am drawn by the need to translate our research findings into therapies to help treat a1 o9 Q, ]" @- p7 w, _( a. R6 ?# E
number of diseases. The promise is great, but we need to deliver, and I believe Current
5 d& O$ v% M) BProtocols in Stem Cell Biology will help tremendously.
7 D# x$ G7 K4 Q- vLeonard I. Zon
4 x) l! o. r! k* F  gCurrent Protocols in Stem Cell Biology7 W& ~! t" X* Q# L
Published online June 2007 in Wiley Interscience (www.interscience.wiley.com).
( k' @$ s. J, ZDOI: 10.1002/9780470151808.scfores1" C, J' u' i4 ^
Copyright C
2007 John% h* e7 {4 e4 J2 D1 C6 }

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