PDF电子书：Protocols for Adult Stem Cells 2010(MiMB 621)

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' N+ {- R* k( |这是2010出版的 Methods in Molecular Biology 系列 vol.621+ h9 N# R1 U0 c. V$ E
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; G- p5 A- M3 Y0 Y7 `8 D0 ?- L) G以下为该书前言：, N2 m7 W& r) ]4 M7 ]( }4 }6 `
Although embryonic stem cells currently enjoy the public limelight and show great promise' K  L: l& P3 R2 U8 j4 q  X) Z" @
for cell based medical therapies, it is the adult stem cells which are responsible for the0 n7 X; O5 _  k. d
body’s natural ability to fight disease, heal and recover, or fail and succumb to various
4 y3 c$ Y4 v. d5 K( Z  Ymaladies. The study of mammalian adult stem cells has surged recently, most likely from a
4 ]5 G- q8 ?: m) Rmaturation of stem cell studies in the classical developmental model organisms and in
% C  _' H& h' a. nhematopoeisis. All the tissues of the body examined so far are generated and regenerated9 b- I6 L+ K$ Y! s6 n& Q( ?7 T
from stem cells, it has been an important first step to adapt or devise new methods to
/ |. R& Y5 E$ E, r: Bidentify and obtain these cells in quantity and purity for further study. Culture techniques1 y  G" }( a3 C; @8 O! j' E% T( `
have been optimized for managing the growth and differentiation of stem cells in vitro; as: m" I0 ~& \  s! Z: ]4 f# E
some stem cells are pluripotent, often the method is to guide the fate of such cells among
5 ]4 X6 E* P% c8 r: athe possible differentiation fates. Much of this work, and that in the classical model organisms,
9 k4 N# r8 }1 }has helped define the aspects of the stem cell environment or niche that are crucial
1 z0 ]( A4 r$ Y8 U9 A3 w! _' wfor both growth and differentiation, and these studies have moved in vivo at increasingly
' k5 f! Z, e) W( phigher resolution. Importantly, the in vivo niche is a current target for bioengineering the7 _8 u) [: `; i4 D
matrix and signaling factors.5 L0 F4 m; Z( I  U& T
Herein, we present methods for studying six types of mammalian stem cells, mammary,
: }8 ]9 o' Z6 ]9 Y. w2 @: o0 Jneural, mesenchymal, endothelial, dendritic, and muscle. We hope the approaches# |1 o; ]' Q- n
described in these chapters will assist you in obtaining, characterizing, and studying these
: ]3 ^$ e0 z7 w# D$ C  Wcells or for adaptation to your favorite stem cells. One final note, should you find the
9 L8 ]0 N- w& ^; finformation herein valuable and it helps you to make great discoveries in the understanding4 ^/ I, F1 b/ Z0 D+ u
of stem cell biology or application, by all means please let us, the authors, know! The
1 m1 P6 ]$ j4 R2 O' j/ ibest way is of course to publish your findings, but falling short of that, please feel free to/ w/ h5 E2 Y8 p7 I/ P
ask questions or clarifications. Especially for this reason, our contact information is provided
$ ^( k9 a' s/ G- `' sat the end of this book.9 ?4 u% u1 E' w* r8 B

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