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本帖最后由 细胞海洋 于 2011-4-27 16:16 编辑
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杂志：Cytotechnology (2011) 63:67–80' W d- L! Z4 p2 @( Y/ B4 u, G
题目 ：Long-term cultures of stem/progenitor cells from lobular and ductal breast carcinomas under non-adherent conditions
1 C3 H. K# ` I y. [0 N* I; m( I7 b作者：Agostina Nardone • Sara Corvigno •, ~& ^1 t# Z1 {, _
Annalisa Brescia • Daniel D’Andrea •
+ L' w P M$ P6 E Gennaro Limite • Bianca Maria Veneziani
8 @( J8 m' B5 c9 n& W8 d文中提到 “To test the ability of LoBS and DuBS cells to form mammospheres, cells were dissociated and seeded, by serial dilution, in ultra low attachment surface 24-well plates in DMEM/F12 plus 0.5% FCS medium (low serum). The final cell dilutions ranged from 10 to 1,000 cells/well. Sphere formation experiments were carried out at least four times per sample.”: P0 N# m. ?8 U
结果如图
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最近养克隆球采用无血清培养基加EGF.FGF.可怎么也不成功。看到这篇文献后有点心动，也想试一试。但血清又有促进细胞分化和贴壁的作用，低血清养出的克隆球和无血清培养出的有可比性吗？! i# G7 L1 v9 V( h1 E/ Q
希望各位可以发表一下自己的意见！: Y' F7 m0 L( E$ y! H6 U+ V$ _. ^
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发表于 2011-3-7 10:12
发表于 2011-3-7 13:56
发表于 2011-3-7 19:59
