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本帖最后由 细胞海洋 于 2011-5-15 23:33 编辑
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Table of Contents7 t& Y4 x; G! w, q
Chapter 1: Plasmids and Their Usefulness in Molecular Cloning5 P4 }) j% r& s
Chapter 2: Bacteriophage and Its Vectors' n! n( x0 e$ z6 _
Chapter 3: Working with Bacteriophage M13 Vectors
: n! p1 e. g. ~+ _5 [0 F$ R; UChapter 4: Working with High-Capacity Vectors
# u; O- p) k; w. ^$ I7 wChapter 5: Gel Electrophoresis of DNA and Pulsed-Field Agarose
* a% f& V' o% N5 M" q' x6 D8 l* m2 g6 hChapter 6: Preparation and Analysis of Eukaryotic Genomic DNA& p i/ W% W5 J2 q7 }+ t, r5 m
Chapter 7: Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells
8 W# p5 k& v. U2 BChapter 8: In Vitro Amplification of DNA by the Polymerase Chain Reaction
! J# t) z3 [! e; ?+ k) X. w- `Chapter 9: Preparation of Radiolabeled DNA and RNA Probes
, e. v0 `3 t! B! K; DChapter 10: Working with Synthetic Oligonucleotide Probes
4 N% W' N% g9 O3 eChapter 11: Preparation of cDNA Libraries and Gene Identification9 U7 l8 \2 p/ G: o! s4 w! _
Chapter 12: DNA Sequencing7 h2 R4 U$ s7 i/ y* I+ o: N
Chapter 13: Mutagenesis7 `4 E7 f( s2 X3 Z) Q
Chapter 14: Screening Expression Libraries$ T3 h" N( A9 q$ v+ s/ C
Chapter 15: Expression of Cloned Genes in Escherichia coli8 L+ t5 m0 H/ l- ^; X& X- }
Chapter 16: Introducing Cloned Genes into Cultured Mammalian Cells+ W) T8 k M# p7 T' T. o; q/ ^
Chapter 17: Analysis of Gene Expression in Cultured Mammalian Cells8 L. y# | M5 z, u; `
Chapter 18: Protein Interaction Technologies
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