ECACC 的细胞培养计数指南--Fundamental Techniques in Cell Culture

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Fundamental Techniques in Cell Culture, Laboratory Handbook, 2nd Edition  v% F( K+ R, b4 q( |3 |

7 {  {3 L0 s& y5 b  Q& W7 kIn response to popular demand the European Collection of Cell Cultures (ECACC), in partnership with Sigma-Aldrich have produced a 2nd Edition of ‘Fundamental Techniques in Cell Culture’. This compact laboratory handbook provides a wealth of information from the sourcing of cell lines, safety and laboratory design to aspects of cryopreservation and quality control. Additionally a series of 10 detailed protocols are provided, which are routinely used in the ECACC laboratories.
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6 `$ X" d8 Y8 I' [8 V# rChapters         Title3 Q; b+ w; n' D9 k# u
1         Introduction
# h& M; O. e1 T* J2         Design and Equipment for the Cell Culture Laboratory0 c7 j' R$ t( g8 G( ?1 j
3         Safety Aspects of Cell Culture! X0 g3 V2 }) R  p# ^# M
4         Sourcing of Cell Lines% o  Y+ u2 R0 N) ?3 j
5         Cell Types & Culture Characteristics
3 H' D) U" d7 w! B5 b5 [6 {6         The Cell Environment (including types of culture medium)
  R- O# \. m& y/ m7         Cryopreservation and Storage of Cells9 y* I! ^! M' l3 P1 ~3 w4 j- t7 l
8         Good Cell Banking Practices8 N' J1 y# G0 k0 ?3 H
9         Quality Control Considerations in Cell Culture
2 v. b- |  W5 _+ ?( |0 z10         Authentication of Cell Lines
) L$ N! u6 u9 y5 ~2 Z11         Alternative Culture Systems* w# N+ B2 `; K
12         Basic Techniques - The “Do’s and Don’ts” of Cell Culture
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Protocols         Title  u( e. @. n" |* u1 |
1         Aseptic Technique and Good Cell Culture Practice8 K3 O4 G9 t3 H
2         Resuscitation of Frozen Cell Lines
& f9 q# q9 T" O, \3         Subculture of Adherent Cell Lines
! R* i0 K- a! B' d/ O4         Subculture of Semi-Adherent Cell Lines
, g9 m, N" D6 U8 Z; h, B% {3 a5         Subculture of Suspension Cell Lines
1 p. b' \0 ~# N" ]6         Cell Quantification
* N: t2 _3 o2 s4 U5 h9 X, E+ ~' ^7         Cryopreservation of Cell Lines4 x9 [8 C7 w/ K) y
8         Testing for Bacteria and Fungi in Cell Cultures
& J6 O# N$ v( v% W" _; L# z9         Testing for Mycoplasma by Culture Isolation- A* N8 ~5 j6 m! U" d
10         Testing for Mycoplasma by Indirect DNA Stain
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Glossary of Terms! D* R( o* l1 a! _  X- \
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