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本帖最后由 细胞海洋 于 2011-9-1 22:04 编辑
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Fundamental Techniques in Cell Culture, Laboratory Handbook, 2nd Edition
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In response to popular demand the European Collection of Cell Cultures (ECACC), in partnership with Sigma-Aldrich have produced a 2nd Edition of ‘Fundamental Techniques in Cell Culture’. This compact laboratory handbook provides a wealth of information from the sourcing of cell lines, safety and laboratory design to aspects of cryopreservation and quality control. Additionally a series of 10 detailed protocols are provided, which are routinely used in the ECACC laboratories.4 X3 A5 j( g. E* w% ~9 n5 C
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Chapters Title# R; k z! K* u" m' G- L
1 Introduction: d. @- `* k; I, Q# v/ t K
2 Design and Equipment for the Cell Culture Laboratory
7 |' e% Q8 L* p, o) I( D; m$ q3 Safety Aspects of Cell Culture
9 P$ n) O8 i& D3 }& B6 g4 Sourcing of Cell Lines
1 K4 a/ F+ G+ G0 U4 b* U! e5 Cell Types & Culture Characteristics6 ]' G: F- z5 } U. V
6 The Cell Environment (including types of culture medium)
% q4 Q( @6 Z/ [2 N8 M2 N' i. a) t7 Cryopreservation and Storage of Cells
% S9 g, B" B9 b! w8 E9 b8 Good Cell Banking Practices
2 b; v& Z6 R' Z. v H9 j9 Quality Control Considerations in Cell Culture
& C5 H& `4 k2 S1 v" B, S4 ? u" i* f10 Authentication of Cell Lines/ N% A9 Q2 ?5 p1 ~, i- A
11 Alternative Culture Systems1 ~8 J* I! D* b4 B( j" T
12 Basic Techniques - The “Do’s and Don’ts” of Cell Culture q( o7 A# M+ u6 }
4 I% n( \3 ~% P1 B* N- SProtocols Title
8 E; F. r7 y5 E- V* h0 x1 Aseptic Technique and Good Cell Culture Practice
0 X/ r4 J$ `$ s4 d! F# L8 O2 Resuscitation of Frozen Cell Lines
3 O+ b: d: z% e! H3 Subculture of Adherent Cell Lines
5 j6 ]; t% i2 F p, i8 K; O7 R4 Subculture of Semi-Adherent Cell Lines5 T; X! H# M! m, E( c7 z& A
5 Subculture of Suspension Cell Lines! X* D- U Q+ c/ @
6 Cell Quantification( H7 X/ o& ?: j9 K5 I
7 Cryopreservation of Cell Lines" Z& L/ G1 @- d3 L
8 Testing for Bacteria and Fungi in Cell Cultures
0 P( J) q* t& T( L H9 @9 Testing for Mycoplasma by Culture Isolation
7 m5 O5 [2 ]2 ]3 ?: t7 V0 ^ E/ n x10 Testing for Mycoplasma by Indirect DNA Stain* F; N( A- l) q4 J
" E* [; X2 U* [# U7 a3 ^: lGlossary of Terms
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发表于 2011-9-1 15:17
