ECACC 的细胞培养计数指南--Fundamental Techniques in Cell Culture

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) V. U" x& Z6 o3 b# N1 QFundamental Techniques in Cell Culture, Laboratory Handbook, 2nd Edition
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In response to popular demand the European Collection of Cell Cultures (ECACC), in partnership with Sigma-Aldrich have produced a 2nd Edition of ‘Fundamental Techniques in Cell Culture’. This compact laboratory handbook provides a wealth of information from the sourcing of cell lines, safety and laboratory design to aspects of cryopreservation and quality control. Additionally a series of 10 detailed protocols are provided, which are routinely used in the ECACC laboratories.
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5 h' F; L) s  n" XChapters         Title
; ]$ q% u9 f; {  R, u9 D, B/ n& R1         Introduction
3 U7 [) s+ A7 p$ l8 a2         Design and Equipment for the Cell Culture Laboratory3 ]- l$ J- q$ f' W1 O* c- I
3         Safety Aspects of Cell Culture- h9 r, ?' J  N1 u
4         Sourcing of Cell Lines
( p$ w0 b+ t5 C+ X5         Cell Types & Culture Characteristics
0 z0 Q! t% \# b; I+ P$ [, K6         The Cell Environment (including types of culture medium)$ Z! D0 b; M& l) A6 T
7         Cryopreservation and Storage of Cells: d5 Z( O* J0 c* |0 e* g
8         Good Cell Banking Practices
) b2 P7 O5 w- o- U9 d9         Quality Control Considerations in Cell Culture
8 V! b; _+ ]* J10         Authentication of Cell Lines
" l7 L( H* K2 [% M6 q) r11         Alternative Culture Systems
  U7 m: N% G, j% A6 L; Y$ v12         Basic Techniques - The “Do’s and Don’ts” of Cell Culture
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Protocols         Title
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2         Resuscitation of Frozen Cell Lines1 N4 L+ o- U  s7 j8 ?1 y2 d( g
3         Subculture of Adherent Cell Lines3 p, d' a" B+ C
4         Subculture of Semi-Adherent Cell Lines: a7 P% J* J- i
5         Subculture of Suspension Cell Lines
9 R7 R# S$ q# C! B0 A( o# I6         Cell Quantification
" R# q* |; @7 U& z- n2 g, E! s7         Cryopreservation of Cell Lines
9 ]: n$ b0 V$ K4 U, n8         Testing for Bacteria and Fungi in Cell Cultures: ~9 L1 W# a, q: y' R$ A$ L
9         Testing for Mycoplasma by Culture Isolation8 ]' a* m' F. [# S: c5 Z
10         Testing for Mycoplasma by Indirect DNA Stain
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Glossary of Terms0 k4 u, |7 m/ A2 r( Z& M$ U1 w$ Y
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