PDF电子书：Recombinant and In Vitro RNA Synthesis

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Recombinant and In Vitro RNA Synthesis4 I% H$ K( ~* A; C6 Z8 N5 a
Contents
0 m# L  I; U4 e$ Q" ~9 [9 GPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v3 g$ m1 |# h1 j9 e0 v" [
Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii4 M& H$ ~( U7 F2 b' `, h& }! \
1 Purification of RNA Expressed In Vivo Inserted in a tRNA Scaffold . . . . . . . . 1
, G2 j7 L& c$ LLuc Ponchon and Frédéric Dardel8 Q8 l. m( g( E5 N1 N9 X
2 Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold. . . . . . . . . 92 e* `( z7 W% z
Luc Ponchon, Geneviève Beauvais, Sylvie Nonin-Lecomte,
8 f8 D3 s6 c& x* Mand Frédéric Dardel* l; u( A" S) w- ]1 B% w- K3 M
3 Preparation of Long Templates for RNA In Vitro Transcription
( ]% k+ i' R4 U7 J9 bby Recursive PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
- T8 `0 [3 ~* ]7 d! g. gJessica C. Bowman, Bahareh Azizi, Timothy K. Lenz, Poorna Roy,1 ]* H) T, C$ m7 n/ ^* l
and Loren Dean Williams! w4 X! _% D% R- s7 R
4 General Protocols for Preparation of Plasmid DNA Template,
8 |3 I$ h. _! I' t6 u, hRNA In Vitro Transcription, and RNA Purification by Denaturing PAGE . . . . 43
1 l1 a* ?  s8 l; P) |Jo L. Linpinsel and Graeme L. Conn5 t- x4 q+ }3 _2 g) @2 ?
5 Preparation of Short RNA by In Vitro Transcription . . . . . . . . . . . . . . . . . . . . 59( X) V; g$ X3 o. x# ]" \+ q4 e: Y
Cheng Lu and Pingwei Li$ {0 B8 B( F5 V/ ], @0 s# S
6 Native RNA Purification by Gel Filtration Chromatography . . . . . . . . . . . . . . 69
! y) u7 C- Z5 ~7 c- ^  K$ I) ]( LEvan P. Booy, Hui Meng, and Sean A. McKenna: P. l, R9 w: E
7 Cis-Acting Ribozymes for the Production of RNA In Vitro  @9 {* S  s$ O4 b, R  h9 E) ?& j
Transcripts with Defined 5′ and 3′ Ends . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
6 E' o# V2 E$ u) F' r* T6 TJohanna M. Avis, Graeme L. Conn, and Scott C. Walker* H( v6 b) v, N9 T
8 Trans-Acting Antigenomic HDV Ribozyme for Production
2 e' k7 D2 z+ R3 c! b5 H% hof In Vitro Transcripts with Homogenous 3′ Ends . . . . . . . . . . . . . . . . . . . . . 99
% J* {' s8 m+ e. }( h0 ^8 KMilena Szafraniec, Leszek Blaszczyk, Jan Wrzesinski,
& y: }" O# o: |/ s# y0 Rand Jerzy Ciesiolka
& ?  ]8 d7 h8 S( B9 Rapid Preparation of RNA Samples Using DNA-Affinity Chromatography
  `! M% c/ N* p4 M3 Iand DNAzyme Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113' u; T( }1 ]0 [
Hae-Kap Cheong, Eunha Hwang, and Chaejoon Cheong0 ^% J' J; d3 A
10 Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNA . . 123" M3 Y: w# L" c; E0 u7 h4 N, A
Geneviève Di Tomasso, Philipe Lampron, James G. Omichinski,: ^) H* f' E: {
and Pascale Legault; f1 C9 r5 J) T9 J  [
11 Affinity Purification of RNA Using an ARiBo Tag . . . . . . . . . . . . . . . . . . . . . . 137; N. H$ j3 `$ V- \: T" M* G
Geneviève Di Tomasso, Pierre Dagenais, Alexandre Desjardins,) G: P. a- Q! d
Alexis Rompré-Brodeur, Vanessa Delfosse, and Pascale Legault
+ Q/ B% j0 z, q" p) L% @7 r12 Plasmid Template Design and In Vitro Transcription of Short RNAs2 y- E) U% `+ ^
Within a “Structure Cassette” for Structure Probing Experiments . . . . . . . . . . 157/ Z8 `1 n8 _' ~& |
13 In Vitro Transcription of Modified RNAs . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171
4 y4 [% r7 f+ L# t2 |Stephanie L. Moon and Jeffrey Wilusz# ~5 [& v! H0 q+ g$ d2 x
14 End-Labeling Oligonucleotides with Chemical Tags After Synthesis . . . . . . . . 181, r+ }' F3 V/ V  n8 Z* t
N. Ruth Zearfoss and Sean P. Ryder
# P; S2 g) M5 z* O) @) W/ L15 High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNA. . . . . . . . 195) _5 U( @& m$ i& {+ Z; |- k
Ya-Ming Hou
* e- p8 }4 y0 ?16 Se-Derivatized RNAs for X-ray Crystallography. . . . . . . . . . . . . . . . . . . . . . . . 213) k$ v" ~: R/ b! \& Y, U
Lina Lin and Zhen Huang
$ z( f1 q& W) Q& F+ a# N17 Biosynthetic Preparation of 13C/15N-Labeled rNTPs for High-Resolution2 M1 ^, w/ o- x# O* L& X, N
NMR Studies of RNAs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 227
4 [4 R- i% @' ?3 f+ O, }# b: N& qLuigi Martino and Maria R. Conte7 K& q  ~* P  {' C
18 Preparative Separation of Ribonucleoside Monophosphates! s4 T( W5 p% }% m! t
by Ion-Pair Reverse-Phase HPLC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 247
/ s. H3 n2 `* z: i5 B# `Pierre Dagenais and Pascale Legault0 g5 I& J5 c  j# Z: u
19 Splint Ligation of RNA with T4 DNA Ligase . . . . . . . . . . . . . . . . . . . . . . . . . 2573 O# c. l; Z3 q7 D0 N$ T  ^
Christopher J. Kershaw and Raymond T. O’Keefe, }" }7 k5 ?3 C4 G0 K1 P
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2714 D$ h0 e+ u. r2 P: G

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