请高手指点这段话是什么意思？谢谢

tianzhang

BglII/KpnI双酶切切除 FLAG标记的 tetrameric wild-type EosFP 中的CMV启动子元件。退火、磷酸化的miniCMV 启动子元件（就是CMV启动子的+51--+77片段）.

tianzhang

This construct was then digested with ZraI to allow insertion of the annealed, phosphorylated and ligated HRE element oligonucleotides. Confirmation of HRE copy number was confirmed by digestion with KpnI/SspI/XbaI andthrough DNA sequencing by the Australian Genome Research Facility .* Q9 k* |4 a/ U* P( a6 S
上述载体构建完毕后使用内切酶 ZraI 消化酶切，然后酶连接 HRE 元件到上述载体上。通过 KpnI/SspI/XbaI酶切确认HRE的拷贝数， Australian Genome Research Facility进行DNA测序

tianzhang

大致意思就是：FLAG tagged tetrameric wild-type EosFP 载体经BglII/KpnI酶切去除CMV启动子，然后插入miniCMV启动子。载体再经过ZraI 酶切后插入HRE元件