- 积分
- 98
- 威望
- 98
- 包包
- 203
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再贡献一个
* m1 ~, f5 ^8 U* b1 |ES medium 100 ml
# y! @" V3 c) x; Z$ ?6 T* l- g& @DMEM (Specialty media, SLM-220-M) 80 ml 8 h& ]# X2 g% X$ g, t+ M* F, j
FBS (Hyclone, SH30071.03) 15 ml , e& D) w n0 l! I1 r
Pen/Strep (100X, Specialty media, TMS-AB-2C) 1 ml (不加也行的,但是建系时要检测支原体)
+ k3 Y/ d1 s' X1 T! D) A! aL-glutamine (100X, Specialty media, TMS-002-C) 1 ml
2 m4 r/ I; @5 |: m1 N% VNonessential amino acids (100X, Specialty media, TMS-001-C) 1 ml
. Q0 R0 @9 N2 a/ O6 L" m8 J9 }Nucleosides for ES cells (100X, Specialty media, ES-008-D) 1 ml
+ R; ~, h L1 X; `. O! e2-mercaptoethanol (100X, Specialty media, ES-007-E) 1 ml
, V: V' _0 H2 ^LIF (ESGRO, GIBCO 13275-029) 10 μl
P/ v; C1 X+ Y; V/ FPD0325901 MEK inhibitor(10mM Stemgent, 04-0006) 10μl (1μM)
% h0 O. \ A9 ?9 LCHIR99021 GSK3βinhibitor(10mM Stemgent, 04-0004) 30μl (3μM)
" |+ n* \( H7 d5 H' t+ m" v- [
- | t4 J) I2 I V1 X0 A2 C: x小鼠ES不用feeder也会长得很好的。如果不加2i也行,就是有些克隆容易分化。 |
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总评分: 威望 + 5
包包 + 15
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