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Handmade Cloned Buffalo (Bubalus bubalis) Embryos Produced from Somatic Cells Isolated from Milk and Ear Skin Differ in Their Developmental Competence, Epigenetic Status, and Gene Expression' z3 f( U. N* ]' q6 H
作者:Jyotsana, B (Jyotsana, Basanti)[ 1 ] ; Sahare, AA (Sahare, Amol A.)[ 2 ] ; Raja, AK (Raja, Anuj K.)[ 2 ] ; Singh, KP (Singh, Karn P.)[ 2 ] ; Singla, SK (Singla, Suresh K.)[ 2 ] ; Chauhan, MS (Chauhan, Manmohan S.)[ 2 ] ; Manik, RS (Manik, Radhey S.)[ 2 ] ; Palta, P (Palta, Prabhat)[ 2 ]! n) S2 R8 C9 l4 C
CELLULAR REPROGRAMMING) y% c# X" K- ?* `0 k' v
卷: 17 期: 5 页: 393-4037 z. r P! v9 S2 _& |9 M) P
DOI: 10.1089/cell.2015.0027; M) H- X& ]$ D
出版年: OCT 1 20158 r; @. n8 P$ U- v. [
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% C4 Y7 f6 F8 v摘要5 L* ^" r4 _( G% l
We compared the cloning efficiency of buffalo embryos produced by handmade cloning (HMC) using ear skin- and milk-derived donor cells. The blastocyst rate was lower (p<0.05) for milk-derived than that for skin-derived embryos, whereas the total cell number and apoptotic index were similar. The global level of H3K9ac was higher (p<0.05) in skin- than in milk-derived cells, whereas the level of H3K27me3 was similar in the two groups. The global level of H3K9ac was similar between milk-derived and in vitro-fertilized (IVF) blastocysts, which was higher (p<0.05) than that in skin-derived blastocysts. The level of H3K27me3 was similar among the three groups. The expression level of IGF-1R and G6PD was higher (p<0.05) in skin- than in milk-derived cells, whereas DNMT1, DNMT3a, and HDAC1 expression level was similar. In the blastocysts, the expression level of DNMT1, HDAC1, OCT4, and CDX2 was higher (p<0.05) in skin-derived than that in IVF blastocysts. The expression level of DNMT3a and IGF-1R, was in the order (p<0.05) skin-derived and IVF>milk-derived blastocysts and that of NANOG was (p<0.05) IVF-> milk-derived>skin-derived blastocysts. The expression level of all these genes, except NANOG, was lower (p<0.05) in milk- than in skin-derived or IVF blastocysts. In conclusion, milk-derived cells can be used for producing HMC embryos of quality similar to that of skin-derived embryos, although with a lower blastocyst rate./ v( v- ?1 i6 ^' f
关键词6 M) G4 [4 h! |$ s( F
KeyWords Plus:NUCLEAR TRANSFER EMBRYOS; PREIMPLANTATION EMBRYOS; METHYLATION; CLONING; EFFICIENCY; INSIGHTS; MAMMALS; CULTURE. s8 s3 Q5 `4 @ m
作者信息
' t7 i0 C. j# ^0 w9 o通讯作者地址: Palta, P (通讯作者)
* H* U( m% w0 Y- P0 N/ i) P显示增强组织信息的名称 Natl Dairy Res Inst, Embryo Biotechnol Lab, Anim Biotechnol Ctr, Karnal 132001, Haryana, India.
% Y \ R5 \" s* M7 N9 H$ x地址:
6 L3 P' x f4 c [ 1 ] Cent Sheep & Wool Res Inst, Anim Biotechnol Sect, Avikanagar 304501, Rajasthan, India$ \# ^0 {: o& O7 P( h8 o" r8 m
显示增强组织信息的名称 [ 2 ] Natl Dairy Res Inst, Anim Biotechnol Ctr, Karnal 132001, Haryana, India% n4 z4 W- h( o7 A2 _+ U5 F
电子邮件地址:prabhatpalta@yahoo.com |
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发表于 2016-1-25 14:09
