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发表于 2009-4-25 10:00 |只看该作者 |倒序浏览 |打印

1 Institute for Research in Biomedicine, CH-6500 Bellinzona, Switzerland

2 Novartis Institutes for Biomedical Research Basel, Neuroscience Research, Novartis Pharma AG, CH-4002 Basel, Switzerland

Abstract& H. r9 a5 g7 w  k  T( w
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Endoproteolysis of the -amyloid precursor protein (APP) by - and -secretases generates the toxic amyloid -peptide (A), which accumulates in the brain of Alzheimer's disease (AD) patients. Here, we established a novel approach to regulate production of A based on intracellular expression of single chain antibodies (intrabodies) raised to an epitope adjacent to the -secretase cleavage site of human APP. The intrabodies rapidly associated, within the endoplasmic reticulum (ER), with newly synthesized APP. One intrabody remained associated during APP transport along the secretory line, shielded the -secretase cleavage site and facilitated the alternative, innocuous cleavage operated by -secretase. Another killer intrabody with an ER retention sequence triggered APP disposal from the ER. The first intrabody drastically inhibited and the second almost abolished generation of A. Intrabodies association with specific substrates rather than with enzymes, may modulate intracellular processes linked to disease with highest specificity and may become instrumental to investigate molecular mechanisms of cellular events.. q$ r- i8 @7 p. O! s

Abbreviations used in this paper: A, amyloid -peptide; AD, Alzheimer's disease; APP, -amyloid precursor protein; BACE, -site APP cleaving enzyme; HEK, human embryonic kidney 293; PS, PBS and permeabilized with 0.05% saponin.. U) z. e2 q' h% j0 l; \) A

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Aging is a major risk factor for Alzheimer's disease (AD) and the number of AD patients will increase in the near future. For this reason, therapeutic treatments against this devastating disease are urgently sought for (Hardy and Selkoe, 2002; Dodel et al., 2003; Cummings, 2004; Mattson, 2004; Tanzi et al., 2004). The amyloid hypothesis holds that generation and deposition of amyloid -peptide (A) are key events driving neurodegeneration in AD (Glenner and Wong, 1984). Immunotherapy involving injection of synthetic A aggregates to elicit neutralizing and aggregate-breaking antibodies and passive A immunization showed promising results in delaying cognitive decline (Younkin, 2001; Haass, 2002), but also underscored the risk of side effects (Pfeifer et al., 2002; Nicoll et al., 2003). Other approaches aim at reducing A generation by inhibiting the secretase activities. -Secretases cleave several substrates and their inactivation appears to interfere with physiologically important signaling pathways (Haass, 2004), but -secretase remains an obvious therapeutic target because its activity can fully be removed in mice by knocking out BACE (-site APP cleaving enzyme) without any obvious toxicity (Luo et al., 2001; Ohno et al., 2004). Inhibitors of BACE are under active study, but the development of specific, cell-permeable drugs that penetrate into the brain remains a challenging task (Kahle and De Strooper, 2003).
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Here, we propose a novel approach to control A production in vivo. The approach is based on intracellular expression of single chain antibodies (intrabodies; Biocca et al., 1990; Bird et al., 1988; Huston et al., 1988; Marasco and Dana Jones, 1998; Lobato and Rabbitts, 2004; Stocks, 2004) that interfere with pathologic endoproteolysis by binding close to the -secretase cleavage site of huAPP (Fig. 1). One intrabody associated within the ER with newly synthesized -amyloid precursor protein (APP). Association persisted during APP transport along the secretory line, protected APP from -secretase cleavage and favored the alternative cleavage by -secretase. This resulted in decreased production of the toxic A peptide and increased production of P3. Another intrabody carrying a carboxy-terminal ER retention signal caused quantitative ER retention and slow disposal of APP, thereby virtually abolishing A production.! F7 L# w% R! O+ _

Results and discussion

The monoclonal antibody 1 (Paganetti et al., 1996) specifically binds to the EFRH tetrapeptide adjacent to the -secretase cleavage site of huAPP (Fig. 1, at position A3-6). 1 was used as template for preparation of two intrabodies named sFv1 and sFv1-KDEL. sFv1 consists of the light and heavy chain variable regions of 1 (132 and 120 residues, respectively) covalently linked by a GGGGS pentapeptide repeated three times. sFv1-KDEL is a variant of the same intrabody carrying the SEKDEL carboxy-terminal residues of BiP/GRP78 to confer ER retention (Munro and Pelham, 1987). The native signal sequence of the light chain was maintained to target the intrabodies to the ER lumen. Liquid chromatography mass spectrometry of secreted sFv1 expressed in human embryonic kidney 293 (HEK) cells revealed that the signal peptide was removed at the consensus site similar to the original 1 antibody (unpublished data).6 d7 a9 Z% J3 x- s

We first determined if sFv1 maintained the capacity of the 1 template to associate with huAPP when expressed intracellularly. HEK cells were transfected for expression of the Swedish variant of huAPP (Mullan et al., 1992) with or without sFv1. 1 d after transfection, cells were metabolically labeled with 35S-methionine and cysteine, chased for 10 min or 2 h, and detergent solubilized. Labeled huAPP was immunoprecipitated from cell lysates with specific antibodies. In mock-transfected cells, huAPP was the only labeled protein isolated (Fig. 2 A, lanes 1 and 2). After a 10-min chase APP is immature (APPi; Mr = 120 kD) in the ER as shown by EndoH sensitivity of its single N-linked glycan (Fig. 2 B). After a 2-h chase most of huAPP was released from the ER and the N-glycan became EndoH resistant (Fig. 2 B). Maturation of huAPP (APPm; Mr = 130 kD) also involves tyrosine-sulfation and O-glycosylation resulting in higher Mr (Weidemann et al., 1989). In cells also expressing sFv1, the intrabody (Mr = 26 kD) coprecipitated with APPi after a 10-min chase (Fig. 2 A, lane 3) and association persisted through the chase (lane 4). Monitoring kinetics of association revealed that the half-time for formation of the intracellular sFv1-huAPP complex was 11 min (Fig. 2 C) and that association between sFv1 and huAPP did not prevent huAPP maturation (Fig. 2 A, lane 4 and Fig. S1, available at http://www.jcb.org/cgi/content/full/jcb.200410047/DC1). The specificity of sFv1 for huAPP was confirmed by the substantial reduction in the amount of sFv1 coprecipitated with a variant of APP characterized by a EFGH versus EFRH mutation in the 1 epitope (Fig. 2 A, RGAPP). Also the parental antibody 1 has strongly decreased affinity for this epitope present in mouse APP.) S% w5 l4 t, e# }
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Next, we coexpressed huAPP with sFv1-KDEL to determine first if this intrabody maintained the capacity to associate with APP, and second to establish if appending an ER-retention sequence to an APP-targeted intrabody also caused retention of huAPP. Cells were metabolically labeled and chased for 10 min and 3 h. sFv1-KDEL had slower electrophoretic mobility than sFv1 (Fig. 3 A). It rapidly associated with newly synthesized huAPP as shown by coimmunoprecipitation after a 10-min chase (Fig. 3 A, lane 3) and association persisted through the chase (Fig. 3 A, lane 4). Unlike sFv1, however, association of sFv1-KDEL with huAPP prevented export of the latter from the ER. In fact, the molecular weight of APP did not increase with progression of the chase (Fig. 3 A, lanes 3 and 4) and the protein failed to acquire EndoH resistance or other posttranslational modifications even after 3 h (Fig. 3 A, EndoH).
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Cytochemical analysis by indirect immunofluorescence revealed that part of huAPP colocalized with the ER marker calnexin (Cnx) and part of it stained clustered regions free of Cnx (Fig. 3 B, squares, panels 1 and 2) but stained with an antibody to the Golgi marker Giantin (Fig. 3 B, panels 3 and 4). Note that all cells are labeled with the markers but only transfected cells are positive for anti-APP. ER and Golgi localization is expected for APP, a secretory protein synthesized in the ER and transported for maturation along the secretory pathway (Weidemann et al., 1989). Expression of sFv1 did not affect the intracellular localization of huAPP, which colocalized in part with Cnx (Fig. 3 B, panels 5 and 6) but was also in perinuclear clusters stained with Giantin (Fig. 3 B, panels 5, 7, and 8). On the other hand, expression of sFv1-KDEL caused retention of huAPP in the ER as shown by exclusion of APP from Giantin-containing structures (Fig. 3 B, panels 9–12). Thus, we generated one intrabody (sFv1) that associated with APP in the ER lumen and remained associated with it during transport along the secretory line. A second intrabody (sFv1-KDEL) was equally efficient and fast to associate with APP, but prevented exit of the target protein from the ER. sFv1-KDEL actually acted as a killer intrabody because it triggered slow disposal of newly synthesized APP in a process that was delayed by MG132, an inhibitor of the cytosolic proteasome (Fig. 3 C).

We next determined if intracellular association of sFv1 and of sFv1-KDEL with APP affected secretase-mediated endoproteolysis resulting in the shedding of the ectodomain of this type I membrane protein. Cells coexpressing huAPP and sFv1 or sFv1-KDEL were metabolically labeled and chased for 10 min or 2 h. To analyze protein secretion, conditioned media were harvested, boiled in sample buffer and analyzed by SDS PAGE. Because of CMV-driven expression, labeled sAPP (and sFv1) are the major secretory products of transfected HEK cells. After 10 min, no labeled ectodomain was secreted (Fig. 4 A, lanes 1, 3, and 5; Fig. S1) as the labeled proteins are still folding in the ER. After a 2-h chase, ectodomain shedding of labeled huAPP had occurred in mock-treated cells (Fig. 4 A, lane 2; Fig. S1). Coexpression of sFv1 significantly reduced (Fig. 4 A, lane 4; Fig. S1), and coexpression of sFv1-KDEL virtually abolished secretase-mediated release of huAPP from cells (Fig. 4 A, lane 6). APP with the mutated 1 epitope served again as specificity control because coexpression of sFv1 did not reduce release of the RGAPP ectodomain in the extracellular media compared with controls (Fig. 4 C).% `" w* H# h, K! A( e

Labeled sFv1 was secreted in the conditioned medium (Fig. 4, A and C, lane 4), whereas virtually no labeled sFv1-KDEL was detected extracellularly (Fig. 4 A, lane 6), as expected for a polypeptide carrying an ER retention signal. Part of the secreted sFv1 was associated and coprecipitated with the secreted huAPP (Fig. 4 B, lane 4) but not with the control protein RGAPP (Fig. 4 C, lane 8). Thus, the complex between the intrabody and huAPP was maintained during secretion and after shedding. From the data described above, we concluded that uncomplexed sFv1 was also released from cells. This is of interest and might have beneficial consequences in case of a therapeutic application of our approach because recognition of the EFRH epitope proved essential for antibodies able to prevent formation or to disassemble preexisting A plaques (Frenkel et al., 1998; Pfeifer et al., 2002). Moreover, passive immunization may rapidly reverse behavioral deficits in mice (Dodart et al., 2002). To establish if in situ release of intrabodies targeted to A exerts protective and/or therapeutic activity awaits further experimentation in an animal model for the disease. Thus, in a series of several independent experiments summarized in Fig. 4 D, we proved that by associating close to the -secretase cleavage site, sFv1 inhibited by >60% the processing and release of the Swedish variant of APP. Addition of a SEKDEL-retention signal led to production of a killer intrabody that retained newly synthesized APP in the ER preventing secretase processing and eventually leading to slow degradation of APP.
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The endoproteolysis of APPSwedish consists mainly in sequential cleavages by - and -secretase releasing the soluble APP ectodomain and the toxic A peptide. A minor alternative endoproteolysis initiated by -secretase releases the soluble APP ectodomain and the peptide P3 (Fig. 1). Therefore, we next determined how coexpression of sFv1 or sFv1-KDEL affected production of the APP metabolites A and P3. APP-derived peptides produced by HEK cells were identified independently by matrix-assisted laser desorption ionization time of flight mass spectrometry (Wang et al., 1996; unpublished data). Here, we determined the peptides by combining immunoprecipitation and immunoblotting with two well-characterized antibodies to the carboxy terminus of A (Paganetti et al., 1996) and electrophoretic comigration with synthetic peptides (Fig. 5).

As demonstrated above using metabolic labeling, steady-state analysis of cell lysates by Western blot confirmed that sFv1 did not interfere with APP maturation (Fig. 5 A, lanes 1 and 2). In contrast, sFv1-KDEL strongly impaired APP maturation (Fig. 5 A, lane 3). Overexpression of APPSwedish produced abundant A as expected for this substrate efficiently processed by -secretase (Fig. 5 B, lane 1). Consistent with the effects observed for the ectodomain shedding, A generation was lowered by coexpression of sFv1 (Fig. 5 B, lane 2) and virtually prevented by sFv1-KDEL (Fig. 5 B, lane 3). In contrast, sFv1 did not affect cleavage of the RGAPP carrying the point mutation in the sFv1 epitope (Fig. 5, A and B, lane 5) and sFv1-KDEL slightly affected RGAPP maturation (Fig. 5 A, lane 6) and did not affect significantly the production of A (Fig. 5 B, lane 6).

Analysis of the ratio A (Mr = 4 kD) versus P3 (Mr = 3 kD) produced in mock-transfected cells and in cells expressing sFv1 (Fig. 5 B, longer exposure, lanes 2 and 3) revealed that sFv1 augmented to a certain extent P3, i.e., shifted APP cleavage from the - to the -site. Thus, association of intrabodies to a sequence adjacent to the -secretase cleavage site interfered with the amyloidogenic processing of huAPP by the -secretase as determined by reduction of release from cells of the APP ectodomain and A. In contrast, the innocuous cleavage by -secretase was slightly favored as shown by increased production of P3.

In summary, we engaged the cellular protein factory, the ER, to produce therapeutic agents inhibiting production of A. By rapidly associating with newly synthesized APP and by escorting it during intracellular transport, sFv1 shielded APP from the pathologic -secretase–mediated cleavage. By appending an ER-retention sequence, we generated an intrabody that retained APP in the ER strongly impairing A production.4 t' C( n' d* z% _, W
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Intrabodies are derived from the virtually unlimited repertoire of antibodies. Unlimited is, therefore, the choice of target molecules. The use of specific intrabodies allowed intervention in an enzymatic process leading to a human disease by targeting the substrate, rather than the modifying enzyme whose activity is at the origin of the disease. By avoiding direct intervention on the enzyme, liabilities for side effects that may be triggered when the enzyme of interest is involved in other physiologic processes are lowered. Our data present a "proof of principle" for an intervention with highest selectivity in pathophysiologic processes in disease.
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Materials and methods( u! i. V% A2 r1 `8 Z- |3 Z+ c

Cell lines and transient transfections

HEK cells were grown in DME supplied with 10% FCS, penicillin, and streptomycin (GIBCO BRL). For transfections, cells plated at 90% confluency were transfected with expression plasmids using Lipofectamine 2000 (GIBCO BRL) according to the manufacturer's instructions for adherent cells. For cotransfections, plasmids for APP and intrabody expression were used in a 1:3 ratio. This DNA ratio makes it unlikely that cells expressing only APP are present in our cultures.
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Metabolic labeling, preparation of cell extracts, immunoprecipitation, and EndoH treatments0 g* ?  s  H, L% _

17 h after transfections cells were starved for 30 min in Met/Cys free medium, pulsed for 10 min with 150 μCi/ml 35S-Met/Cys in starvation medium and chased with DME supplemented with 5 mM of cold Met/Cys. Cell extracts were prepared by solubilization in 800 μl/dish ice-cold 2% CHAPS in Hepes buffer saline, pH 6.8, containing 20 mM ice-cold N-ethylmaleimide and protease inhibitors (HBS). Postnuclear supernatants were prepared by a 10-min centrifugation at 10,000 g and analyzed by reducing SDS-PAGE as described in Molinari et al. (2003). 12% polyacrylamide gels were used, with the exception of 8% gels in the EndoH analysis (Figs. 2 and 3) and for Fig.5 A. Analysis of A and P3 in Fig. 5 B was done on 13% Tris/bicine gels (Klafki et al., 1996). Immunoprecipitations were performed using antibodies and protein A beads (Sigma-Aldrich) and 1–4-h incubations in a cold room. The immunoprecipitates were washed three times with HBS/0.5% CHAPS and resuspended in sample buffer for SDS-PAGE. Gels were exposed to BioMax (Kodak) films and scanned with an Agfa scanner. For EndoH treatment, APP was immunoprecipitated from the cell extracts with specific antibody and denatured before incubation for 1 h at 37°C with 1 mU of EndoH (Roche Molecular Biochemicals). For immunoblotting, protein in cell extracts or immunoprecipitates were separated by reducing SDS-PAGE and transferred to Immobilon P membranes (Millipore) before blotting with specific antibodies (Paganetti et al., 1996).

Indirect immunofluorescence microscopy

For indirect immunofluorescence, HEK cells were plated for 24 h on alcian blue-treated glass coverslips, washed twice with PBS, and fixed at RT for 20 min in serum-free medium Hepes containing 3.7% formaldehyde. Cells were washed twice in serum-free medium Hepes, twice with PBS and permeabilized with 0.05% saponin (PS) for 15 min. Cells were incubated with primary antibody diluted in PS for 45 min, washed 15 min with PS, then incubated with conjugated secondary antibody diluted in PS for 30 min. Cells were rinsed with PS and water and mounted in Mowiol.+ u, A8 d( ~# f

Microscopic images were collected at RT using a microscope (model E-800; Nikon) equipped with a 60x/1.4 Plan Apo objective, filter cubes for CFP and YFP fluorescence, and a camera (Q-Imaging), controlled by Openlab 3.5 software. Image cropping and adjustment were accomplished using Photoshop (Adobe).
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Online supplemental material  y1 x1 d& i& p- ~

Acknowledgments6 v9 f- I$ a. p0 j) v

We thank L. Bolliger, D. Neri, G. Noseda, and S. Monti for helpful comments; C. Barske and D. Bleckmann for excellent technical assistance., w9 ^9 l, }" j& t" K3 i) D/ A
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M. Molinari is supported by grants from Max Cloetta Foundation, Foundation for Research on Neurodegenerative Diseases, Synapsis Foundation, Swiss National Center of Competence in Research on Neural Plasticity and Repair, Telethon and Swiss National Science Foundation.0 C* y' f2 ^# p: U! M  O6 R

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