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Cell Stem Cell2009年4月3日的一篇文献，希望对大家有帮助8 A5 }3 n+ s2 Q, G- U" [8 s
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Cell Stem Cell 4, April 3, 2009
0 q% }: b+ E( `, A9 fMolecules that Promote or Enhance. w& N; V' [* [0 ]
Reprogramming of Somatic Cells
U: ^3 M) D9 W& @' _to Induced Pluripotent Stem Cells: q, q# i+ @/ I R
Bo Feng,1 Jia-Hui Ng,1 Jian-Chien Dominic Heng,1 and Huck-Hui Ng1,2,*
$ `7 i5 G) \& s" G1 @, o0 S8 O# X, d1Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672, Republic of Singapore& S6 d f* v# o# U3 H; Q: M) f
2Department of Biological Sciences, National University of Singapore, Singapore 117543, Republic of Singapore
; a' d) V2 k/ S* ~4 |*Correspondence: nghh@gis.a-star.edu.sg/ e2 a' p) H4 r" w* k }9 j
DOI 10.1016/j.stem.2009.03.005
, K8 `$ y% O6 nReprogramming of somatic cells to induced pluripotent stem cells (iPSCs) can be achieved by viral-mediated
* w5 ?! ]# q4 C8 l- W1 |+ J) Etransduction of defined transcription factors. Moving toward the eventual goal of clinical application, it is
! i; i: Z+ r& u- A" D4 x& k* N* f! onecessary to overcome limitations such as low reprogramming efficiency and genomic alterations due to viral
- ?* S8 e9 R1 ^: p; c8 ? eintegration. Here, we review recent progress made in the usage of genetic factors, chemical inhibitors,0 }' ` e+ Z, J1 @' W8 k: y+ n
and signaling molecules that can either replace core reprogramming factors or enhance reprogramming
/ v( i0 v, [1 ]& J+ aefficiency. Current iPSC studies will provide a paradigm for the combinatorial use of genetic factors and
3 h3 c9 r M( J3 Ychemicals for the broader applications to alter cellular states of potency.
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abbr_bd5620d537f49c8cee29d8a5f5bfca43.pdf
(292.33 KB)
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发表于 2009-4-27 13:21
发表于 2009-5-6 14:21
