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[干细胞与细胞生物学类]
PDF电子书：Lentivirus Gene Engineering Protocols (Methods in Molecular Biology (Hard
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Lentivirus Gene Engineering Protocols (Methods in Molecular Biology (Hardcover))
ISBN: 1588290913 Title: Lentivirus Gene Engineering Protocols (Methods in Molecular Biology (Hardcover)) Author: Publisher: Humana Press Publication Date: 2003-05 Number Of Pages: 314 Average Amazon Rating: Download Details: I myself have dreamed of this book for a long time. I'd like to share it with all our EBooksclubers, especially for those who are doing basic sciences of biomedicine once I got it. Special thanks to Ebooksclub.org, who provides such a terrific platform for sharing joys, happiness and surprise among our Ebooksclubers! Methods in Molecular Biology, vol.229 Maurizio Federico has assembled a panel of outstanding experimenters to detail all the theoretical and practical aspects of lentivirus vector-based gene transfer. The authors demonstrate lentivirus vector production with methods for recovering appropriate producer cells, for producing and titrating lentivirus-containing supernatants, and for detecting transduced cells. The applications of lentivirus vector engineering to different cell types include coverage of lymphocytes, dendritic cells, hematopoietic stem and progenitor cells, mesenchymal stem cells, hippocampal neurons, cardiomyocytes, as well as airway epithelia, corneal cells, and retinal pigment. Also advanced are applications in which genetic material is directly inoculated with lentivirus vectors in such experimental animal models as mouse, rat, and rabbit. [attach]814[/attach]
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