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第一篇摘要，全文还没有搞到，等等
* G2 ~- n& d2 W% x! [Multiple roles for Sox2 in the developing and adult mouse trachea5 `9 B- r3 ` h1 n' @
Jianwen Que1, Xiaoyan Luo1, Robert J. Schwartz2 and Brigid L. M. Hogan1,*
+ {. _( c% M: ^ T. T, N( B1 Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.
$ A9 q. d; E* l c) n( o: u2 Center for Molecular Development and Disease, Institute of Biosciences and Technology, Texas A&M University System Health Science Center, Houston, TX 77030, USA.
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/ h, l) C9 Z# {" v! d* Author for correspondence (e-mail: b.hogan@cellbio.duke.edu)
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/ B! l- x' `2 R3 L5 q' k' f9 nAccepted 31 March 2009
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6 V6 \- p, J# I* J: ZThe esophagus, trachea and lung develop from the embryonic foregut, yet acquire and maintain distinct tissue phenotypes. Previously, we demonstrated that the transcription factor Sox2 is necessary for foregut morphogenesis and esophagus development. We show that Sox2 is also required for the normal development of the trachea and lung. In both the embryo and adult, Sox2 is exclusively expressed in the epithelium of the trachea and airways. We use an Nkx2.5-Cre transgene and a Sox2 floxed allele to conditionally delete Sox2 in the ventral epithelial domain of the early anterior foregut, which gives rise to the future trachea and lung buds. All conditional mutants die of respiratory distress at birth, probably due to abnormal differentiation of the laryngeal and tracheal cartilage as a result of defective epithelial-mesenchymal interaction. About 60% of the mutants have a short trachea, suggesting that the primary budding site of the lung shifts anteriorly. In the tracheal epithelium of all conditional mutants there are significantly more mucus-producing cells compared with wild type, and fewer basal stem cells, ciliated and Clara cells. Differentiation of the epithelium lining the conducting airways in the lung is abnormal, suggesting that Sox2 also plays a role in the differentiation of embryonic airway progenitors into specific lineages. Conditional deletion of Sox2 was then used to test its role in adult epithelium maintenance. We found that epithelial cells, including basal stem cells, lacking Sox2 show a reduced capacity to proliferate in culture and to repair after injury in vivo. Taken together, these results define multiple roles for Sox2 in the developing and adult trachea.
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5 _0 @6 K4 r, U6 _* W1 JKey words: Sox2, Foregut development, Trachea, Epithelial-mesenchymal interaction, Epithelial differentiation, Injury-repair, Mouse |
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发表于 2009-6-16 00:15
发表于 2009-6-16 08:03
发表于 2009-6-16 09:47
