- 积分
- 112
- 威望
- 112
- 包包
- 55
|
Three-dimensional type I collagen gel system for the study of osteoblastic metastases produced by metastatic prostate cancer.
; \ `6 x2 `% e& H- ^* V% ~) p8 N* r/ s- `4 v* P
Koutsilieris M1, Sourla A, Pelletier G, Doillon CJ.4 c+ z. k |, t( r2 p
Author information
P/ S4 k4 k; ?1 Q QAbstract) U0 [6 A+ K' ]% R/ Z5 Z
We developed a three-dimensional type I collagen gel cell culture system that allows coculturing of human MG-63 osteoblast-like cells and various human cancer cells. Inoculation of human PC-3 metastatic prostate cancer cells into this type I collagen gel containing human MG-63 osteoblast-like cells produced an osteoblastic-like reaction that presented as an increased number of MG-63 cells and increased density of type I collagen around MG-63 cells adjacent to inoculated PC-3 cells by microscope analysis. Under identical experimental conditions, inoculation of cell-free medium, human KLE endometrial adenocarcinoma cells, and Calu-1 lung cancer cells did not produce this blastic-like reaction. In situ hybridization documented the uniform expression of insulin-like growth factor I (IGF-I) and of urokinase-type plasminogen activator (uPA) mRNA in MG-63 and PC-3 cells separately cultured in this substrata. The uniform expression of uPA was also documented by immunocytochemistry using a monoclonal and a polyclonal antihuman uPA antibody. The relative expression of uPA was higher in PC-3 cells than in MG-63, KLE, and Calu-1 cancer cells. We conclude that this novel cell culture system may become a useful model to study the pathophysiology of the osteoblastic reaction in vitro.* K2 L) Y! ^/ [
PMID: 7863832 DOI: 10.1002/jbmr.5650091120 |
|