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本帖最后由 细胞海洋 于 2009-8-3 11:48 编辑
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求助文献一篇:Differentiation of neuroepithelia from human embryonic stem cells" h6 m5 b1 c9 I6 @4 c& Y
Methods Mol Biol. 2009;549:51-8* k" H) a# |7 n0 [
Xia X, Zhang SC.' M$ `5 r+ g/ g
We describe the method for efficiently differentiating human embryonic stem cells to neuroepithelial cells in a chemically defined condition. The protocol was established based on the fundamental principle of in vivo neuroectodermal development. The temporal course, morphological transformation, and shift in gene expression of our neuroepithelial differentiation closely resemble those occur during in vivo development. In particular, the primitive neuroepithelial cells generated by this protocol can be further induced into neuronal and glial cells with forebrain, mid/hind brain, and spinal cord identities and targeted transmitter phenotypes.
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2 O% a, E# y7 d, gPMID: 19378195 [PubMed - indexed for MEDLINE]- ]- `: Q/ `+ ~; H8 t, p
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细胞海洋注:已解决,文献在回复6楼 |
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