Nature Methods：人类胚胎干细胞分化的全新追踪法

niyou

2009-9-3 20:44:46 70
& g: ^8 `  _" I; f* x+ y
$ h8 b  ^% v( J4 W' Y' [+ N3 l( kNature Methods：人类胚胎干细胞分化的全新追踪法9 l0 \% s, d$ {9 T
% ]9 N( Z) [2 D# Q/ R
人类胚胎干细胞能分化为不同的体细胞。报告细胞系（Reporter cell lines） 能优先分化出研究着所需要的细胞类型。Nature Methods最新报道了，Elefanty和他同事发现的一种人类胚胎干细胞系-ErythRED。ErythRED受β球蛋白基因调控区序列控制。ErythRED表达仅局限于成熟的红细胞，因此可以用于检测体外红细胞分化，并确定体外胚胎干细胞转化为红细胞路径。& R4 u  |9 c7 n

" G; ~3 g% l8 e8 q1 E原文出处：7 D/ B3 ~: k" a" v

  m' ~( R( o  j: P7 ^1 H3 C* x' gNature Methods 6, 659 - 662 (2009) | doi:10.1038/nmeth.1364
8 ^2 x9 z. ~# K6 `$ v% {+ Q8 X4 F# G/ z
ErythRED, a hESC line enabling identification of erythroid cells! q: V0 {; S0 D8 h# I
Tanya Hatzistavrou1, Suzanne J Micallef1, Elizabeth S Ng1, Jim Vadolas2, Edouard G Stanley1,3 & Andrew G Elefanty1,3( t/ ]% ^% Y8 K. t
& }- l6 d3 G  t" g$ I9 a- z
A human embryonic stem cell (hESC) line that enabled globin-expressing cells to be easily recognized would facilitate optimization of erythroid differentiation in vitro and aid in the identification of hESC-derived erythroid cells in transplanted animals. We describe a genetically modified hESC line, ErythRED, in which expression of RFP, controlled by regulatory sequences from the human -globin locus control region, is restricted to maturing erythroid cells.$ x5 h& t. I  d8 N0 u+ B" W

1 B; h" ?! l2 ]) ~- iMonash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia.7 b2 g7 l! L# ]; r$ `; `& U
Cell and Gene Therapy Group, Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia.4 l2 |' }" ~3 _1 \2 s
These authors contributed equally to this work.

wy200810

是不是特异性的启动子也可以完成这项任务啊，特异性的启动子，只在主要在一些细胞内才能表达，用这种启动子做成慢病毒或者其他的载体再带上荧光标记，也可以起到示踪的效果吧！$ i7 w( |# k" R7 n
http://www.systembio.com/index.php?id=product_41

kim22222

谢谢

edisonlou

谢谢，学习

可怜的孩子

学习了