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2009-9-3 20:44:46 70+ L0 ~8 R; V9 y- j# o& l* @9 I
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Nature Methods：人类胚胎干细胞分化的全新追踪法) m& f/ `4 y* a. `1 x2 H- ^
* }/ y( S% M7 G! W/ I! h" q人类胚胎干细胞能分化为不同的体细胞。报告细胞系（Reporter cell lines） 能优先分化出研究着所需要的细胞类型。Nature Methods最新报道了，Elefanty和他同事发现的一种人类胚胎干细胞系-ErythRED。ErythRED受β球蛋白基因调控区序列控制。ErythRED表达仅局限于成熟的红细胞，因此可以用于检测体外红细胞分化，并确定体外胚胎干细胞转化为红细胞路径。: |8 [+ d' ?: o$ P
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原文出处：
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& w# t; H2 b: M4 m' R$ tNature Methods 6, 659 - 662 (2009) | doi:10.1038/nmeth.1364: I9 m' f/ U! E5 Y2 d' }! v
! ^3 D- r( @. d% A. \/ ?ErythRED, a hESC line enabling identification of erythroid cells7 M5 z; N( u7 e0 Z. G/ K( k
Tanya Hatzistavrou1, Suzanne J Micallef1, Elizabeth S Ng1, Jim Vadolas2, Edouard G Stanley1,3 & Andrew G Elefanty1,3
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A human embryonic stem cell (hESC) line that enabled globin-expressing cells to be easily recognized would facilitate optimization of erythroid differentiation in vitro and aid in the identification of hESC-derived erythroid cells in transplanted animals. We describe a genetically modified hESC line, ErythRED, in which expression of RFP, controlled by regulatory sequences from the human -globin locus control region, is restricted to maturing erythroid cells.
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Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia.
) R- e3 e9 z+ e+ @: UCell and Gene Therapy Group, Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia.% M! u6 t: t3 S
These authors contributed equally to this work. |
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发表于 2009-9-8 17:04
发表于 2009-9-9 10:16
