ES分化不通过EB

happy20091981cn

不知道有没有做ES的同仁，诱导ES分化有没有不通过EB的方案？EB只是模拟胚胎发育的过程，有没有不通过EB来诱导的？个人感觉形成EB后，刺激因子不容易直接与细胞接触，我想看一些因子最早期对ES分化的影响，或者改良法减少做EB的时间（最经典的是2+5的方案，这是心肌细胞分化的方案）。不知道有没有相关的文献，园子的高手能推荐下么？谢谢

痞子的烟头

你可以查paper， 诱导分化常见也就
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2 EB
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gemstone

Direct differentiation of human embryonic stem cells to hepatocyte-like cells exhibiting functional activities
8 r3 ]) t6 Z' k8 SAuthors: David C Hay, Debiao Zhao, Arlene Ross, Ramkumar Mandalam, Jane Lebkowski, Wei Cui( D* k6 w8 Y$ Z# s: b! X; S+ I9 c
Impact factor: 2.94, Cited half life: 3.8, Immediacy index: 0.52
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Journal: Cloning and Stem Cells3 A5 v2 Z7 Z( J4 K3 E! D; X8 V

" j; S. M* q  W8 ?8 L2 hThe utilization of human hepatocytes for biomedical research, drug discovery, and treatment of liver diseases is hindered by the limited availability of donated livers and the variability of their derived hepatocytes. Human embryonic stem cells (hESCs) are pluripotent and provide a unique, unlimited resource for human hepatocytes. However, differentiation of hESCs to hepatocytes remains a challenge. We have developed a multistage procedure by which hESCs can be directly differentiated to hepatocyte-like cells without embryoid body formation and the requirement of sodium butyrate. The hESC-derived hepatocyte-like cells (HLCs) exhibited characteristic hepatocyte morphology, expressed hepatocyte markers, including alpha-fetoprotein, albumin, and hepatocyte nuclear factor 4alpha, and possessed hepatocyte-specific activities, such as p450 metabolism, albumin production, glycogen storage, and uptake and excretion of indocyanine green. Hepatocyte growth factor was found to play a positive role in promoting hepatocyte differentiation. Our differentiation system has shown that hESCs can be differentiated to hepatocyte-like cells capable of executing a range of hepatocyte functions. Therefore, it presents a proof-of-principle of potential applications of using the hESC-derived hepatocytes. Additionally, the hESC-derived HLCs provide a unique model to study the mechanisms involved in human hepatocyte differentiation and liver function.: w, q3 B3 N) C7 H9 U4 w9 r
Cloning and stem cells. 01/02/2007; 9(1):51-62.

edisonlou

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chendongbo338

可以与基质蛋白共培养，例如四型胶原，最有利于研究信号通路；也可以与基质细胞共培养，例如OP9。

lucillelee

通过添加诱导因子

zhaoqiaoshi

回复 1# happy20091981cn
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( o0 G* t# [; Z* O/ K    关注下 长长见识

sun_happone

新手询问:EB到底是什么呀？我刚开始这一块，什么都不知道呢。

恋水鱼

通过trypsin消化成单克隆 然后单克隆培养 再诱导分化 也很不错 分化效率很高的

dilal

回复 8# sun_happone ! W% a- N, I/ b/ d
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& `  C7 J8 Z/ e    EB就是拟胚体，模拟胚胎发育的过程，是ESC心肌分化的一种培养方法。