造血干细胞单克隆形成有哪些检测指标

dadaviery

实验中有一部分是检测目的基因对造血干细胞单克隆形成能力的影响，发现形成的大多都是小克隆。除了计算克隆形成的大小、细胞的数量，还有什么别的检测指标吗？谢谢啦

louise33

Depends on which types of colony assay that you are using. If you analyse CFU-C (i.e. Myeloid progenitors), you can distinguish CFU-GEMM, CFU-GM and BFU-E by the color as well (since BFU-E is red/slight red in color due to the presence of hemogloblin). You can take the colonies to make cytospin followed by geimsa staining, by this, you can distinguish the morphology of the colonies as well

deron

通过对其表面标志分子（可查阅相关工具书或文献）的检测来确定是否为造血干细胞单克隆，检测方法的具体细分还有排除法逆推法等。这是最经典的方法。