PDF电子书：Arabidopsis Protocols

lifescience1

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Preface
1 K# f+ m7 e2 h7 M/ ?For several decades, Arabidopsis thaliana has been the organism of choice in the6 G, `) e& L) T- e6 H( ^8 }: G
laboratories of many plant geneticists, physiologists, developmental biologists, and
: D4 U9 u3 e! w& K- ~, l" pbiochemists around the world. During this time, a huge amount of knowledge has been
' ^1 A; ]9 k: t: H6 ~acquired on the biology of this plant species, which has resulted in the development of- J) I5 c% w% j* |3 ~3 V5 ^
molecular tools that account for much more efficient research. The significance that# e) f! y: x4 o3 P0 B% R
Arabidopsis would attain in biological research may have been difficult to foresee in4 ?( V, `! k! _+ C. D2 V; Z
the 1980s, when its use in the laboratory started. In the meantime, it has become the& N( b# [9 G0 M* P4 t  X
model plant organism, much the same way as Drosophila, Caenorhabditis, or mouse  t$ j- E/ ^2 Z& y4 s! v5 V
have for animal systems. Today, it is difficult to envision research at the cutting edge of' n. e3 }: `1 U6 T
plant biology without the use of Arabidopsis.
5 A0 P8 E" y$ z. F5 BSince the first edition of Arabidopsis Protocols appeared, new developments have. |9 k. U: y& ~9 v' r
fostered an impressive advance in plant biology that prompted us to prepare Arabidopsis
. Z% p6 ~! ~) _Protocols, Second Edition. Completion of the Arabidopsis genome sequence offered for8 h5 S% W# F; ^$ c* w  u
the first time the opportunity to have in hand all of the genetic information required for! f: _) _# m' a- _1 l3 S
studying plant function. In addition, the development of whole systems approaches that' y  d8 X, j4 y2 z3 i
allow global analysis of gene expression and protein and metabolite dynamics has" b+ B) r) i# c7 i0 k# F# |
encouraged scientists to explore new scenarios that are extending the limits of our: t! Y8 y0 ^' K3 X6 F+ n
knowledge. These advances will eventually lead to an understanding of how this complex,) s& E( d- r' A. S+ C9 F
multicellular organism works, how it copes with the particularities of a sessile life
! a% m& g. A7 ~; l$ A# w' s6 Cstyle, and how these strategies compare with those developed in other organisms.8 E/ q3 J! k7 ]
While conceiving this second edition of Arabidopsis Protocols, our aim remained as it- b! b- p/ T3 u/ N6 E5 g
was in the first: the book should provide both experienced researchers and beginners in  _9 F7 u7 F9 a
the field of plant biology with a comprehensive set of up-to-date protocols covering the
/ U" x7 Z# f4 s! o- cmany methods developed for work with this species. Readers should have little problem
1 m; e3 _+ e$ y/ @understanding the general design or the specific details of the experimental protocols.
9 H5 H* T' Q! U/ KNevertheless, we recommend reading the protocols in advance, before bench work is
7 d6 K) n; Z5 Z4 J/ P% X  L& Jstarted. Although a few chapters in this edition have been maintained and updated, most3 G6 A; }* ]$ S5 U8 s9 Y& W" V
are new and describe technologies that have only very recently been developed in+ l3 o. o; @2 ?3 @/ ?  d6 J" I
Arabidopsis. We hope that Arabidopsis Protocols, Second Edition will continue as a, ~' e0 i% {9 s
standard reference for laboratories working with Arabidopsis and other plant species.0 Q0 N% ]( L: A5 b. S
Julio Salinas
/ ]/ ^! C8 M/ v; ~4 EJose J. Sanchez-Serrano& _, ?4 |; H9 E% e# q& T7 l
因为有些大，我把它分成了2部分。下载之后PDF软件合并一下就可以成整本了哈。

细胞海洋

好资料 下次不用分成两部分 论坛附件最大限制30mb

lifescience1

7 d; G. C7 `) b" N9 B( T; ]
1 b5 G& n7 L$ t7 |3 B回复 2# 细胞海洋
5 r) E' ?" A8 G+ }$ O1 u0 f4 E' G. h4 Q  \7 w/ B

! `4 I5 {0 O- f! t8 w! J* {( `. h! M好的。谢谢版主
& j0 \2 ~; r6 Y; F  难怪我在上传  GENE IX 的时候 两个附件分别为60Mb   上传了发帖之后帖子里没有显示附件。

gggarfield

谢谢

lifescience1

回复 4# gggarfield
8 W! _4 b+ N: T6 {2 I. H  ^3 }不用客气哈。大家一块分享。

cz200203

老了点，不过还有点参考价值

lifescience1

恩。是有点过时哈。

llingzu

真的很感谢

lghe44

正需要，楼主雪中送炭

zech_zang

好东西，谢谢分享