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本帖最后由 细胞海洋 于 2010-1-17 21:00 编辑 * m. I- l! J( B' e& l" x( q6 b( ]/ S
" W+ C9 C5 N: F! |3 \5 w1 yPreface, N: H- |8 G, f# f8 N1 @, w3 k8 @
For several decades, Arabidopsis thaliana has been the organism of choice in the; S9 E5 z$ b7 P2 }
laboratories of many plant geneticists, physiologists, developmental biologists, and
; |# F1 Z' o( c$ nbiochemists around the world. During this time, a huge amount of knowledge has been, E M/ h8 h1 T( y* S# y
acquired on the biology of this plant species, which has resulted in the development of
& Z5 r( |. u2 F9 _% Q4 Zmolecular tools that account for much more efficient research. The significance that% R7 W1 t0 |2 j. c k5 v
Arabidopsis would attain in biological research may have been difficult to foresee in8 k, G4 k4 H+ H7 u6 M
the 1980s, when its use in the laboratory started. In the meantime, it has become the
0 e! D' ?0 r5 t+ q) t v7 G' t7 gmodel plant organism, much the same way as Drosophila, Caenorhabditis, or mouse
+ q3 K8 X9 v* w D% q* x* [have for animal systems. Today, it is difficult to envision research at the cutting edge of
/ M: [' O) m3 S% ~; W; Cplant biology without the use of Arabidopsis.
, l$ ^8 B6 O. N6 H# KSince the first edition of Arabidopsis Protocols appeared, new developments have
( X8 ^4 G7 {% T" W8 d! efostered an impressive advance in plant biology that prompted us to prepare Arabidopsis8 H: E, u4 |: T m
Protocols, Second Edition. Completion of the Arabidopsis genome sequence offered for
" e# h( `& s( P" g7 Pthe first time the opportunity to have in hand all of the genetic information required for9 ^) C0 U8 R5 H. x' z, p
studying plant function. In addition, the development of whole systems approaches that
I( p) P1 P: U, Zallow global analysis of gene expression and protein and metabolite dynamics has4 ?" d5 g ?$ V7 U
encouraged scientists to explore new scenarios that are extending the limits of our
% \2 a% ~- m9 D+ E% vknowledge. These advances will eventually lead to an understanding of how this complex,5 v# n2 N# Q: I: M* G
multicellular organism works, how it copes with the particularities of a sessile life
]6 y! m4 _& Zstyle, and how these strategies compare with those developed in other organisms.) L2 Z1 ?) |0 O5 P- I0 X
While conceiving this second edition of Arabidopsis Protocols, our aim remained as it
2 |( Y- r, |" E" f9 z$ pwas in the first: the book should provide both experienced researchers and beginners in
6 R' o7 U5 A2 T, T) hthe field of plant biology with a comprehensive set of up-to-date protocols covering the
; E w# f }. C2 O% H! P# n/ amany methods developed for work with this species. Readers should have little problem9 k% E3 [, w j$ J1 i
understanding the general design or the specific details of the experimental protocols.
o, c4 I8 A9 b8 i2 G, _; }" QNevertheless, we recommend reading the protocols in advance, before bench work is9 O8 h, m3 ?. C# i: j
started. Although a few chapters in this edition have been maintained and updated, most
7 t0 X/ @) j9 H; i4 @! C4 B6 j( {& Yare new and describe technologies that have only very recently been developed in: o4 u% C& `1 z0 i, I
Arabidopsis. We hope that Arabidopsis Protocols, Second Edition will continue as a, W$ B/ e& L2 |* n" \! h: N J
standard reference for laboratories working with Arabidopsis and other plant species.
/ L" _/ M' r! O: L6 M7 sJulio Salinas
$ ^/ t* w- R9 T0 _6 v5 EJose J. Sanchez-Serrano
4 n( ?( b$ {1 [: q因为有些大，我把它分成了2部分。下载之后PDF软件合并一下就可以成整本了哈。 |
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发表于 2010-1-14 23:36
发表于 2010-1-14 23:57
