还得麻烦大家

uyunbilig

我校小气的Springer和Interscience都没给买，哎。还得麻烦大家 找一篇 Defined conditions for neural commitment and differentiation
3 \8 p/ r/ h9 B8 J  e* U谢谢各位了！* o  v" G- L0 [6 X8 x- U3 x' M
6 F, a) t1 m! r( h# v
Methods Enzymol. 2003;365:327-41.
' `8 N1 F. [, e: e+ [1 ~& y$ u6 L; [6 i4 A. h' \5 ~
Defined conditions for neural commitment and differentiation.
2 J* \" N7 J+ O7 z0 UYing QL, Smith AG.
& S* i- E8 L$ D; e7 h0 S- j4 J5 V& f
( G& n- K3 J# sInstitute for Stem Cell Research, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh, Scotland, EH9 3JQ, UK.
' K4 A! E/ @# O% w1 c/ _9 D% o- G( a/ e! j" j1 [+ v
The efficiency of monolayer differentiation establishes that commitment of ES cells to a neural fate needs neither multicellular aggregation nor extrinsic inducers. The entire process by which pluripotent ES cells acquire neural specification can be visualized and recorded at the level of individual colonies. Furthermore this simple culture system is amenable to cellular and molecular dissection, promising to yield new insights into the mechanism underlying neural determination in mammals and perhaps to deliver the goal of "directed" homogeneous differentiation of ES cells.