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本帖最后由 细胞海洋 于 2010-2-28 07:45 编辑 5 L8 ?( h& C/ H6 P, w' c1 O% y
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The scope of epigenetics is vast, and an exhaustive analysis of all of the
a7 W8 g# u1 Rtechniques employed by investigators would be unrealistic. However, this `; i& c1 [: R; K, O
volume of Methods in Molecular Biology™ covers three main areas that should
u2 A m/ c3 K2 l0 O$ y& Tbe of greatest interest to epigenetics investigators: (1) techniques related to
) B- s1 L9 p1 Oanalysis of chromatin remodeling, such as histone acetylation and methylation;
: D( V0 R- S# p3 O. V: W, o(2) methods in newly developed and especially promising areas of epigenetics3 X$ p3 o ~6 \6 c; v, V
such as telomere position effects, quantitative epigenetics, and ADP
& {, j6 L" a' u2 Z9 U8 u9 W7 T% z3 jribosylation; and (3) an updated analysis of techniques involving DNA
) ~( u A9 i Amethylation and its role in the modification, as well as the maintenance, of& @/ ^. Y- l, c' u' Q1 U
chromatin structure.2 v' G }' w; r0 v% }$ f( d
The protocols presented in Epigenetics Protocols are intended to provide
' }5 V, |( _, }8 h4 Jinvestigators with a contemporary set of tools that can be applied to research in; q0 r+ c. [# T5 d4 I: ]; ]' }0 s' A
the field. These tools include recent breakthroughs in epigenetic analysis such
4 a8 g G& \0 w8 p. \& I# Nas techniques for determining changes in native chromatin, methods of
. M- E( z$ J5 p# \microarray analysis as applied to epigenetics, and methylation-sensitive singlestrand- @0 @: Q0 c% n; a
conformation techniques. These methods, as well as many others
. J( x P6 }1 {. ?provided in Epigenetics Protocols, have strong potential for further facilitating
8 I7 O; o& p1 x6 v" @# @ O2 fdevelopments in this promising and rapidly developing field.
' \4 u, l' ]) r7 Z9 PTrygve O. Tollefsbol |
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