造血干细胞筛选的标志？

catheryne

各位大侠，筛选纯化的造血干细胞到底应该用什么标志？CD34+、CD117+、CD133+?

iseeyou1210

actually the fetal HSC and adult HSC are different.; L2 v5 q1 M2 n! ]) m. `
for fetal HSC, they are lin- mac1+ c-kit+ sca1+ cd34+
1 J* L2 v, u" o* p, l- e1 vfor quiscient adult HSC, they are lin- mac1-c-kit+ sca1+ cd34- or low.& B0 c- I) A# A. G" b$ _  J& t, q! B
however, I think for normally research lin-c-kit+ sca1+ are enough.
/ D: Z9 f' `: U+ F, b" {the state of cd34 is under debate.

fish_zbw1985

回复 2# iseeyou1210
0 g7 [" Z7 F" `2 L- ?. z鼠和人的分选标记不太一样，你说的这都是鼠的标志吧

ppcl2

Mouse HSC : CD34lo/-, SCA-1+ , Thy1.1+/lo, CD38+, C-kit+, lin- . j$ A5 J1 \3 B
Human HSC : CD34+, CD59+, Thy1/CD90+, CD38lo/-, C-kit/CD117+, lin-

catheryne

多谢各位的回答，受益匪浅。咨询了一些美天妮的技术人员，小鼠干细胞一般采用SCA-1+或CD117+，分别代表不同的发育阶段，SCA-1阳性标志出现的更早一些。

bjchen789

human：- \0 t5 L2 R- S7 n( c, G) O
stem and progenitor cell (HSC/HPC) research has contributed a great deal to the understanding and harnessing of hematopoieisis for use within therapeutic research applications.
# s' A  K, f4 v6 n( n; _ HSCs/HPCs using CD34, CD133, CD117 MicroBeads or the Lineage Cell Depletion Kit8 C- z1 e6 z4 g9 w& Y8 `2 ]
Lineage-committed progenitor cells（系定向分化祖细胞） using CD33 or CD71 MicroBeads

bjchen789

isolation of mouse hematopoietic stem and progenitor cells from bone marrow. These cells are negative for lineage commitment markers (lin–) and positive for Sca-1 and CD117 (c-kit). For the separation of long-term repopulating hematopoietic stem cells (LTR-HSCs), the CD105 MultiSort Kit (PE) can be used in conjunction with the Anti-Sca-1 MicroBead Kit (FITC).
- ]4 P% ^1 s- _3 y" ]4 a' z( u* t# ^% L; |
CD117 (c-kit) and Sca-1 expression is also found on a variety of stem cells from bone marrow with nonhematopoietic differentiation potential, as well as on tissue-specific stem cells. Sca-1 is expressed by stem and progenitor cells found in cardiac tissue1, mammary gland tissue2, skin, lung, muscle, and testis.

chenmay

谢谢

laolifeidao999

谢谢。

louise33

For adult murine stem cells, LSK cells were the basic requirement. However, nowadays, laboratories, which aim at carrying out single-cell transplantation into the mice, use a lot of additional markers to isolate a purer population. E.g CD34 (CD34-), CD150 (CD150+), CD49b (CD49b-) or even adding some Hoechst dye to look for side population (because primitive cells are able to pump out the dye).
! a8 G% W# H$ e1 ]  LFor human HSC from Cord blood, there are not so many markers that you can choose from. Typically CD34+CD38-CD90+ contains HSC, but still a hetergeneous propulation. Some labs use Rho123 (more primitive cells are Rho123-/low) or ALDH as well