文献求助，谢先！

wuxiao101325

【求助】求助英文文献1篇 , Y' z* S7 t& f+ b  X
【篇号】: 1( a6 m- u# t) J* c& X2 ~+ |6 e
【作者】Feng Ma! I7 i3 F' S' ^4 H2 G
【文章标题】 A Novel Method for Efficient Production of Multipotential Hematopoietic Progenitors from Human Embryonic Stem Cells by Co-Culture with Murine Fetal Liver-Derived Stromal Cells.
( c2 ]& p7 u; p6 `3 z5 G$ `" o【期刊名，年份，卷（期），起止页码】Blood (ASH Annual Meeting Abstracts) 2005 106: Abstract 4214
9 c" ?4 ?6 [" o  M/ O% w0 t【全文链接】http://abstracts.hematologylibra ... bstract/106/11/42146 N& t% l1 _' K5 t' l! }/ V
【求助者联系方式】wuxiao101325@tom.com

sxlc

http://g.zhubajie.com/urllink.php?id=8639377nowojm9xgi3opguj

wuxiao101325

回复 2# sxlc 0 K: T- _. J  R2 }2 z
非常感谢朋友的热心帮助，但是你给的这篇文献不是我求助所要的文献，不管怎么说，还是非常感谢！

wuxiao101325

不好意思让各位热心胞友费心了，我求助的这篇文献其实是会议摘要，原本就没有全文的，再次感谢大家的热心帮助！

oldmac7

楼主找的是这篇:
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8 p$ ?; M: {/ V3 P3 }" c0 `9 ZA Novel Method for Efficient Production of Multipotential Hematopoietic Progenitors from Human Embryonic Stem Cells by Co-Culture with Murine Fetal Liver-Derived Stromal Cells.
9 i! S7 N3 H5 g5 s) {Feng Ma*, Dan Wang*, Sachiyo Hanada*, Hirohide Kawasaki, Yuji Zaike*, Toshio Heike*, Toshio Kitamura, Tatsutoshi Nakahata* and Kohichiro Tsuji ) n0 \/ l  f- [! @- X
1 Division of Cellular Therapy, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan; 2 Department of Laboratory Medicine, Research Hospital, Institute of Medical Science, University of Tokyo, Japan and 3 Depatment of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto, Japan. $ l& T, [' @7 N% H9 t, Y

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Abstract
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/ B+ f( F4 r0 EHuman embryonic stem cells provide a unique tool to study early events occurring in the development of human embryonic hematopoiesis, and their totipotent capability indicates a potent clinical application based on the cellular therapy and the evaluation of drug effects on hematopoietic and blood cells. To achieve efficient production of hematopoietic cells from human embryonic stem cells, we attempted to reproduce the circumstance surrounding embryonic hematopoietic cells in vitro. Since fetal liver is the predominant source of hematopoietic and blood cells in mammalian embryogenesis, we established stromal cells from mouse fetal liver at days 14 to 15 of gestation. In the co-culture of human embryonic stem cells with the established stromal cells, a number of hematopoietic progenitors were generated at around day 14 of co-culture, and this hematopoietic activity was highly enriched in the cobble stone-like cells under the stromal layer. Most of the cobble stone-like cells collected expressed CD34 and contained a variety of hematopoietic colony-forming cells, especially multilineage colony-forming cells, at a high frequency. The multipotential hematopoietic progenitors in the cobble stone-like cells produced all types of mature blood cells, including adult type hemoglobin-synthesizing erythrocytes and tryptase and chymase-bouble positive mast cells in the suspension cultiue with a cytokine cocktail. The developed co-culture system of human embryonic stem cells should offer a novel source for hematopoietic and blood cells applicable to cellular therapies and drug screening.