关于细胞培养过程中污染的问题！

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最近的培养过程中，细胞生长和之前没有问题，但是，总是被检测出细菌污染和支原体污染，尤其是最近一次检测，检测出来了一种非常见的革兰氏阳性菌，具体的配图在下面，还望各位老师帮忙解释一下，就是为什么和之前的培养没任何区别，细胞生长也没有区别，生长周期和没有被检测出污染长的情况一致，就是反复被检测出污染，麻烦各位老师了！

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cohnella formosensis     这个是最近检测出来的菌，因为我用手机发表的，不知道如何传照片。

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摘要
2 h3 \3 r* L/ w& _8 Z0 h从台湾紫花苜蓿根际分离到一株革兰氏阳性、孢子形成、好氧、棒状、木聚糖溶菌，命名为CC-苜蓿-35(T)。基于16S rRNA基因序列的系统发育分析表明，菌株CC-苜蓿-35(T)属于科尼拉属(Cohnella)。菌株CC-苜蓿-35(T)对16S rRNA基因序列的同源性为95.3%，与该属类型菌株(Cohnella抗温菌DSM 17683(T)的同源性为97.4%~93.6%。CC-苜蓿-35(T)与刺参22296(T)的DNA-DNA杂交值为37.7%±1.7%(倒数值为55.7%±3.0%)。主要的细胞脂肪酸为iso-C16：0和anteiso-C15：0。极性脂质组成为二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、赖氨酸-磷脂酰甘油、三种未识别的磷脂和三种未识别的氨基磷脂。主要呼吸醌为MK-7，DNA G+C含量为58.3mol%.菌株CC-苜蓿-35(T)含有中二氨基丙酸作为细胞壁肽聚糖中的主要二胺酸。根据与耐高温菌DSM 17683(T)相一致的极性脂和脂肪酸谱，结合附加的基因型、表型和化学分类学特征，提出了一种新的种，命名为Cohnella formosensis sp.nov.，命名为Cohnella formosensis sp.nov.，命名为Cohnella formosensis sp.。菌株为CC-苜蓿-35(T)(=JCM 18405(T)=BCRC 80428(T).

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Cohnella formosensis sp. nov., a xylanolytic bacterium isolated from the rhizosphere of Medicago sativa L.! K6 v' c. z# U& J1 `- z
Hameed A , Hung MH , Lin SY , Hsu YH , Liu YC , Shahina M , Lai WA , Huang HC , Young LS , Young CC .
1 _8 U3 B3 N0 h8 G3 iAbstract; X0 H/ C* _$ k8 l# {
A Gram-positive, spore-forming, aerobic, rod-shaped, xylanolytic bacterium designated strain CC-Alfalfa-35(T) was isolated from the rhizosphere of Medicago sativa L. in Taiwan. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain CC-Alfalfa-35(T) was affiliated to the genus Cohnella. Strain CC-Alfalfa-35(T) shared 95.3 % pairwise 16S rRNA gene sequence similarity to the type strain of the type species of the genus Cohnella (Cohnella thermotolerans DSM 17683(T)) besides showing a similarity of 97.4-93.6 % with other recognized species of the genus Cohnella. The DNA-DNA hybridization value between CC-Alfalfa-35(T) and Cohnella thailandensis KCTC 22296(T) was 37.7 % ± 1.7 % (reciprocal value, 55.7 % ± 3.0 %). Predominant cellular fatty acids were iso-C16 : 0 and anteiso-C15 : 0. The polar lipid profile constituted diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, lysyl-phosphatidylglycerol, three unidentified phospholipids and three unidentified aminophospholipids. The major respiratory quinone was MK-7 and the DNA G+C content was 58.3 mol%. Strain CC-Alfalfa-35(T) contained meso-diaminopimelic acid as the major diamino acid in the cell-wall peptidoglycan. Based on the polar lipid and fatty acid profiles, which were in line with those of C. thermotolerans DSM 17683(T), coupled with additional distinguishing genotypic, phenotypic and chemotaxonomic features, strain CC-Alfalfa-35(T) is proposed to represent a novel species within the genus Cohnella, for which the name Cohnella formosensis sp. nov. is proposed. The type strain is CC-Alfalfa-35(T) ( = JCM 18405(T) = BCRC 80428(T)).

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求助啊) P9 N! ]# C# {# H9 V9 P5 V

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细菌污染和支原体污染一般都会影响到细胞生长的，如果生长没有问题，可能的原因是细胞生长的环境和营养成分没有受到影响，可能你加了抗生素抑制了细菌的生长速度，没有抢走细胞的营养成分吧。还有就是可能你们检测假阳性，可以送外面检测一下试试，因为有的仪器就经常会出假阳性的。