良性瘤里也能分到肿瘤干细胞（附原文）

hcoohboy

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Br J Cancer. 2009 Jul 21;101(2):303-11. Epub 2009 Jun 30.- Q6 a' B  U' N% P1 v) {1 G8 y% F
Isolation of tumour stem-like cells from benign tumours.
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# w% [7 J; e  c% {2 QXu Q, Yuan X, Tunici P, Liu G, Fan X, Xu M, Hu J, Hwang JY, Farkas DL, Black KL, Yu JS.  A8 d" q% M# E/ M5 Q. y

' F3 N  ?& k% I0 uDepartment of Neurosurgery, Cedars-Sinai Medical Center, Los Angeles, CA, USA.: ]! R% Y" j8 q# R- _
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+ C" Q! o# h# [/ @9 q) oBACKGROUND: Cancerous stem-like cells (CSCs) have been implicated as cancer-initiating cells in a range of malignant tumours. Diverse genetic programs regulate CSC behaviours, and CSCs from glioblastoma patients are qualitatively distinct from each other. The intrinsic connection between the presence of CSCs and malignancy is unclear. We set out to test whether tumour stem-like cells can be identified from benign tumours. METHODS: Tumour sphere cultures were derived from hormone-positive and -negative pituitary adenomas. Characterisation of tumour stem-like cells in vitro was performed using self-renewal assays, stem cell-associated marker expression analysis, differentiation, and stimulated hormone production assays. The tumour-initiating capability of these tumour stem-like cells was tested in serial brain tumour transplantation experiments using SCID mice. RESULTS: In this study, we isolated sphere-forming, self-renewable, and multipotent stem-like cells from pituitary adenomas, which are benign tumours. We found that pituitary adenoma stem-like cells (PASCs), compared with their differentiated daughter cells, expressed increased levels of stem cell-associated gene products, antiapoptotic proteins, and pituitary progenitor cell markers. Similar to CSCs isolated from glioblastomas, PASCs are more resistant to chemotherapeutics than their differentiated daughter cells. Furthermore, differentiated PASCs responded to stimulation with hypothalamic hormones and produced corresponding pituitary hormones that are reflective of the phenotypes of the primary pituitary tumours. Finally, we demonstrated that PASCs are pituitary tumour-initiating cells in serial transplantation animal experiments. CONCLUSION: This study for the first time indicates that stem-like cells are present in benign tumours. The conclusions from this study may have applications to understanding pituitary tumour biology and therapies, as well as implications for the notion of tumour-initiating cells in general.
/ x. G6 ^2 `: Z4 q2 }---------------谁发一下全文，我下载不了
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* s, V; `2 N$ @+ w: j2 e/ a/ u海洋注：7楼原文 感谢pujixing 提供

marrowstem

原文中那不叫肿瘤干细胞 ，叫tumour stem-like cells。

hcoohboy

回复 2# marrowstem 1 i$ S4 F% M' J( i& J

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' M7 h# ]# T, }; k    是的，老外还是很严谨的：）

prettybear

回复 2# marrowstem 7 X- {' j" y4 `/ e7 Y7 i" X
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    可能不只是谨慎，也是为了以示区别。

hcoohboy

回复 4# prettybear 3 H" W* U  B7 q8 z7 q

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% x* ]- v/ e9 I( T: I7 T    噢？有可能~可惜我下不了全文，谁能找到全文传一下，可以好好读一下

wang_kai1212123

新的角度 新的出发点  ~~~学习了

pujixing

原文请参阅这里的PDF文档。希望对大家有所帮助。

jiandong2001

谢谢七楼的原文

zhiduoduo

这个

gaowei1980

谢谢pujixing提供的原文。