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本帖最后由 sunyucheng 于 2010-5-12 21:56 编辑
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3 R* b8 v! o( v. U( i- yGenerating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4.9 ~6 x o: w+ m4 k2 g
G. Grant Welstead, Tobias Brambrink, Rudolf Jaenisch
w$ z. h2 D9 b: F/ T! ]" GWhitehead Institute for Biomedical Research, Massachusetts Institute of Technology.
1 x* K; l1 P3 q2 q+ P0 S# Z/ [内容:0:10 Cellular Reprogramming10
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3:30 Introduction2103 X$ g9 ] `1 w- ^0 h9 W
Q$ b, b: R8 b5 K) r/ e z2 c4:32 Transfecting HEK293T Cells and Purifying Lentivirus272% n7 M8 L) U3 W: H8 Q- o( ?
8 }2 |1 P7 f0 T! F* l: b6:08 Infection of MEFs with Lentivirus368! |/ Z9 o5 W0 E/ I1 a% p
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8:56 Picking up the iPS Colonies536
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9 t1 B6 v0 c! j11:15 Expansion of iPS Cells675
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12:32 Conclusion752
0 D: A1 B1 Z& J9 A7 Y6 _2 N4 Ahttp://www.jove.com/index/Details.stp?ID=734 |
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