以色列耶路撒冷哈德萨医学中心：悬浮液可用于大规模培育胚胎干细胞

细胞海洋

该发现为干细胞治疗的推广扫清了障碍% Z9 h; B5 f& [. ~* R1 ]
2010年05月13日 来源： 科技日报 作者： 郑晓春7 R- z' }( |' |! Q- \
　　本报特拉维夫5月10日电（记者郑晓春）以色列耶路撒冷哈德萨医学中心的科学家宣布，他们用悬浮液培育胚胎干细胞取得突破，向大规模培育胚胎干细胞用于医学治疗的目标前进了一步。   ^6 b; _; R; N: f$ e$ c
　　胚胎干细胞是人体的全能细胞，有发育为各种人体组织和器官的潜力，对治疗帕金森氏症等许多疑难疾病，以及修复和更换受损的细胞组织有重要意义。但由于胚胎干细胞来源有限，加上目前用培养基培育胚胎干细胞的方法工作强度大，难以实现大量培育，使胚胎干细胞治疗在应用上面临很大障碍。3 K& @: z# N7 ~7 L5 o) w  N
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　　针对这一问题，以色列科学家研发了一种利用特殊的悬浮液培育胚胎干细胞的新方法。实验显示，胚胎干细胞可以在这种悬浮液中继续生长，而不会分化为其他类型的细胞，培育过程也比原有方法更为简便，从而为胚胎干细胞的大规模培育及其治疗的普及开辟了一条新途径。
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; }. j5 `/ W" Y9 m　　负责这项研究的本杰明·鲁宾诺夫教授表示，胚胎干细胞治疗有广泛的应用前景。现在，美国科学家已向美国食品和药物管理局提出将胚胎干细胞用于脊髓损伤治疗的申请。在以色列，距离胚胎干细胞治疗的临床试验也不是很远了。研究人员希望，在未来两年内，能够开始用胚胎干细胞修复老年性眼部分子退化的临床试验。不过，在进行临床试验前，他们还需要对胚胎干细胞的治疗效果进行深入研究，以确保植入人体内的胚胎干细胞能够生长为所需的人体组织，而不会产生癌变。

uyunbilig

希望有人附上原文！

jianlindunev

我找了一下，好像就是这篇文章。Derivation, propagation and controlled differentiation of human embryonic stem cells in suspension
/ e( Q( ~' @$ i1 G% r) I% z' h4 ZDebora Steiner,Hanita Khaner,Malkiel Cohen,Sharona Even-Ram,Yaniv Gil,Pavel Itsykson,Tikva Turetsky,Maria Idelson,Einat Aizenman,Rita Ram,Yael Berman-Zaken& Benjamin Reubinoff
! F0 e; q0 Y0 {3 Y* F3 h6 q% v( EAffiliations Contributions Corresponding author Journal name:
. ?0 L' z. ~' j4 t8 |* X  V) ~Nature Biotechnology : c) {- }6 g" t" E
Volume:
' Y; y! W1 I+ [, R; o( N) v8 N8 n28, , p" ?. n  q6 m
Pages: 1 q1 J/ t0 p) I) _  B/ j
361–364
6 C8 r+ O* @- F+ w6 r* H5 \3 ZYear published:
4 d2 N- R" \" M* F1 p) M(2010)
* j9 Z% F& t! c( g4 Z1 C$ C( ~DOI:
$ W4 J9 y8 S3 U  ~' M, h6 Fdoi:10.1038/nbt.1616 6 B" h- U' n+ f; q- M7 U. Q# z, B
Received 24 July 2009 Accepted 16 February 2010 Published online 28 March 2010
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Share/bookmarkConnotea Cite U Like Facebook Twitter Delicious Digg Undifferentiated human embryonic stem cells (hESCs) are currently propagated on a relatively small scale as monolayer colonies1, 2, 3, 4, 5, 6, 7. Culture of hESCs as floating aggregates is widely used for induction of differentiation into embryoid bodies8. Here we show that hESC lines can be derived from floating inner cell masses in suspension culture conditions that do not involve feeder cells or microcarriers. This culture system supports prolonged propagation of the pluripotent stem cells as floating clusters without their differentiation into embryoid bodies. HESCs cultivated as aggregates in suspension maintain the expression of pluripotency markers and can differentiate into progeny of the three germ layers both in vitro and in vivo. We further show the controlled differentiation of hESC clusters in suspension into neural spheres. These results pave the way for large-scale expansion and controlled differentiation of hESCs in suspension, which would be valuable in basic and applied research.2 ~( ]; _& S; }- {4 _9 T' V/ m! i5 {2 J
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还有一篇相关的。

jianlindunev

Stem Cell Rev. 2010 Apr 30. [Epub ahead of print]1 h9 Z% _5 Y+ t
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Suspension Culture of Undifferentiated Human Embryonic and Induced Pluripotent Stem Cells.! }$ q+ F; m3 I! E" M& W  [, E6 U# w- q+ ^
Amit M, Chebath J, Margulets V, Laevsky I, Miropolsky Y, Shariki K, Peri M, Blais I, Slutsky G, Revel M, Itskovitz-Eldor J.
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Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, and the Sohnis and Forman Families Stem Cell Center, the Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, 31096, Israel.
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Abstract
3 e% c( X, K1 c  i0 jAlongside their contribution to research, human embryonic stem cells (hESC) may also prove valuable for cell-based therapies. Traditionally, these cells have been grown in adhesion culture either with or without feeder cells, allowing for their continuous growth as undifferentiated cells. However, to be applicable in therapy and industry they must be produced in a scalable and controlled process. Here we present for the first time a suspension culture system for undifferentiated hESC and induced pluripotent stem cells (iPSC), based on medium supplemented with the IL6RIL6 chimera (interleukin-6 receptor fused to interleukin-6), and basic fibroblast growth factor. Four hESC lines cultured in this system maintained all ESC features after 20 passages, including stable karyotype and pluripotency. Similar results were obtained when hESC were replaced with iPSC from two different cell lines. We demonstrate that the IL6RIL6 chimera supports the self-renewal and expansion of undifferentiated hESC and iPSC in suspension, and thus present another efficient system for large-scale propagation of undifferentiated pluripotent cells for clinical and translational applications.