Nature：ES 和 iPS细胞中基因表达的比较（附原文）

ydj19830

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专题：Nature系列
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1 W$ Z) t% C8 i# aiPS细胞（诱导多能干细胞）在多大程度上相当于胚胎干细胞（ES细胞）是一个并没有惟一答案的问题。一些研究报告称，与ES细胞相比，数百个基因在iPS细胞中异常表达，而iPS细胞能够通过四倍体胚胎互补生成全为iPS细胞的小鼠来满足关于发育潜力的最为严格的测试之一。+ w5 ^$ v" {* {& @

6 B3 w# c+ c+ P* }: i' z! G1 g为了用最少量的涉及因子来回答这一问题，Stadtfeld等人对在遗传上相同的小鼠ES 和 iPS细胞中的基因表达进行了比较。他们发现，mRNA 和 microRNA总体表达模式是无法区分的，少数转录体和少于20个由12qF1染色体上一个印记基因簇编码的microRNA除外。iPS细胞的发育潜力取决于基因在这个点上是被关闭（沉默）还是处于活跃状态。
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生物谷推荐原文出处：
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* T& K% a% H; INature doi:10.1038/nature09017
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Aberrant silencing of imprinted genes on chromosome 12qF1 in mouse induced pluripotent stem cells0 l6 z) p4 @. Z8 d
Matthias Stadtfeld,Effie Apostolou,Hidenori Akutsu,Atsushi Fukuda,Patricia Follett,Sridaran Natesan,Tomohiro Kono,Toshi Shioda& Konrad Hochedlinger
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Induced pluripotent stem cells (iPSCs) have been generated by enforced expression of defined sets of transcription factors in somatic cells. It remains controversial whether iPSCs are molecularly and functionally equivalent to blastocyst-derived embryonic stem (ES) cells. By comparing genetically identical mouse ES cells and iPSCs, we show here that their overall messenger RNA and microRNA expression patterns are indistinguishable with the exception of a few transcripts encoded within the imprinted Dlk1–Dio3 gene cluster on chromosome 12qF1, which were aberrantly silenced in most of the iPSC clones. Consistent with a developmental role of the Dlk1–Dio3 gene cluster, these iPSC clones contributed poorly to chimaeras and failed to support the development of entirely iPSC-derived animals (‘all-iPSC mice’). In contrast, iPSC clones with normal expression of the Dlk1–Dio3 cluster contributed to high-grade chimaeras and generated viable all-iPSC mice. Notably, treatment of an iPSC clone that had silenced Dlk1–Dio3 with a histone deacetylase inhibitor reactivated the locus and rescued its ability to support full-term development of all-iPSC mice. Thus, the expression state of a single imprinted gene cluster seems to distinguish most murine iPSCs from ES cells and allows for the prospective identification of iPSC clones that have the full development potential of ES cells.3 Z: g" p# X3 E# o

. s$ \$ e$ B# f1 xhttp://www.bioon.com/biology/cell/442801.shtml

yanjie11678

全文( |' Z) ~- A- v( A4 A

fguw

iPS细胞能够通过四倍体胚胎互补生成全为iPS细胞的小鼠,?
1 ?$ _0 i& L, C2 s$ p- h比较熟悉该内容的同志,能不能解释一下?

念想儿

回复 3# fguw
: h$ y+ ]3 V7 f/ S0 _据我了解的是这样。两个二倍体胚通过电融合生成四倍体胚，然后两个四倍体胚将IPS细胞夹在中间，类似于三明治结构。这样如果IPS细胞发育为后代，就可以认为这个后代是完全由ips发育而来，因为四倍体胚只参与胚外组织的发育。之后就可以用分子生物学的检测手段鉴定IPS产生的小鼠了。例如甲基化情况，嵌合体的生成，及后代可育能力的检测等等。详细的我推荐你看看周琪的那篇paper，里面介绍的很详细。希望对你有帮助。

fguw

谢谢,因为没有这方面背景, 以前有哥们似乎也告诉过我类似的知识,不过,听过就忘记了, 嘿嘿,希望这次记住,

ydj19830

回复 2# yanjie11678
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    谢谢提供全文~ ~

saberly

谢谢啊

wuxiao101325

谢谢分享

sea200k

谢谢分享

alioli

多谢了