人牙周膜干细胞的初步鉴定及体外成骨诱导

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摘要：目的：体外培养、鉴定人牙周膜干细胞（periodontal ligament stem cells，PDLSCs）并定向诱导分化为成骨细胞，探讨人PDLSCs的多向分化潜能：方法：体外分离、培养人牙周膜细胞，待细胞达一定量后用有限稀释法进行克隆化培养，筛选鉴定牙周膜干细胞（PDLSC），矿化诱导培养21d后检测钙结节形成情况、ALP活性，免疫细胞化学检测骨涎蛋白（BSP）、Ⅰ型胶原表达，RT—PCR检测ALP、BSP mRNA表达。结果：人PDLSCs体外诱导培养21d后可见钙结节形成，成骨细胞相关蛋白及mRNA均阳性表达。结论：人PDLSCs在体外诱导条件下具有成骨潜能。: T( k' b% m7 F. Y: M
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KeyWord：human periodontal ligament stem cells; cell cloning; induced differentiation& d1 ^2 j  p, X5 G( v7 u/ g! @0 O/ I

, p  ~& B# W) E6 c关键词：牙周膜干细胞 细胞克隆 诱导分化
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分类号：Q813.12 Q813.5[著者标引]文献标识码：A文章编号：1005-4979（2008）05-0318-05: ~" S9 e! {3 ^  D: J, S  \3 p
Human Periodontal Ligament Stem Cells Differentiation into Osteoblasts in vitro ZHEN Lei, LIU Hong-wei Department of Oral Medicine, Hospital of Stomatology, Tongji University, Shanghai 200072, China
* Y" {" h/ Q( n; k4 P1 R6 O& H+ QAbstract：Objective： To isolate and purify the human periodontal ligament stem cells（PDLSCs） and investigate the differentiation potentials of PDLSC into osteoblasts in vitro. Methods： PDLSCs were isolated and cultivated. PDLSCs of passage 2 was plated at density of 5×10^3/cm^2. At 80% confluence, the medium was changed to an inducing medium. After 21 days of induction, the results were evaluated by Alizarin red S staining, ALP activitv test, immunohistochemistry and RT-PCR. Results： PDLSCs were cultivated and differentiated in vitro with active function, which were manifested by Alizarin red S staining and ALP activity test. Positive expression of collagen Ⅰ , BSP, ALP were confirmed by immunohistochemistry and RT-PCR. Conclusion： PDLSCs can be induced into osteoblasts in vitro. PDLSCs have the capability of multilineage differentiations.4 v- U$ C8 P: ?
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KeyWord：human periodontal ligament stem cells; cell cloning; induced differentiation7 q% R- N( b2 @

luwei8157

自从南加州大学施松涛教授发现牙周膜干细胞以来，PDLSC的研究得到广大牙周再生修复的研究者的关注。如何利用这一细胞再生牙周组织，微环境的作用非常大，各位同仁都是使用什么诱导模式？

barton_nyd

看看啊，呵呵

隆锦华

学习学习

tkpss18

thank you for sharing!!

uuyuq

学习

open1230

Thank you for sharing!!

qianqianlaile

多谢啦
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