If your experimental needs call for a viral preparation of in vivo grade quality, store the supension at 4 °C for no longer than 48 h.+ b8 f; T& _" T7 L
Filtered supernatants can be stored at 4 °C for up to 3 d before proceeding with concentration,or aliquoted and stored at –20 °C (for periods < 1 month) or –80 °C (for periods > 1 month). Minimize freeze-thaw cycles (ideally <3).