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本帖最后由 细胞海洋 于 2010-7-1 20:26 编辑 4 F& a: K- _, O: l" E- l; H
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Alexandra L. Joyner) U) C2 G7 O% H" Y
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Editorial Reviews- K3 g6 n: a1 v; k
Review
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"An introduction for beginners and a bench reference generally to the techniques now available by which scientists can create essentially any mutation in the germline of mice by using recombination and embryonic stem cells (ESC). Genetic researchers, most from the US but also representing Britain, Canada, and Germany, cover principles and practice in mammalian cells, site-specific recombination in cells and mice, producing targeted ESC clones, producing chimeras by blastocyst and morula injection of targeted ESC, producing and analyzing ESC aggregation, gene trap strategies, and classical genetics and gene targeting. The appendix lists suppliers and contact information but not products."--SciTech Book News4 d9 Q$ F. [) a% e6 a8 D
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Product Description6 U# P4 z9 q: w- ^4 V8 F3 q8 m8 |
This updated and revised edition is well illustrated with over 60 figures and nearly 20 photographs. With over 50 protocols, it is useful for postgraduate engineers, molecular biologists, and biomedical researchers/ g7 w/ B" Y. L6 M2 k0 A4 Q1 J' S, v
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# d4 u2 {$ }2 t& o* ]4 F8 qTable of Contents5 f5 P) K) ~. I! a1 f5 A& w1 i
Preface : j l/ { |/ |6 J# F
List of Contributors
' p3 r6 F# e7 o: _, uAbbreviations
/ u2 Z1 J( k: `9 z+ D1. Gene targeting, principles, and practice in mammalian cells , Paul Hasty, Alejandro Abuin, and Allan Bradley
' ~: c e( ?- \* ~6 E2. Site-specific recombination in cell and mice , Susan M. Dymecki
5 Q; q! Q* Z5 G' Z( d3 H# C Z( o3. Production of targeted embryonic stem cell clones , Michael P. Matise, Wotjek Auerbach, and Alexandra L. Joyner
) I' X3 K* u: [7 f6 N4. Production of chimeras by blastocyst and morula injection of targeted ES cells , Virginia Papaioannou and Randall Johnson
( B" A8 r% k" z @5. Production and analysis of ES cell aggregation chimeras , Andras Nagy and Janet Rossant 9 b- R/ Z! G' t1 n p" @
6. Gene trap strategies in ES cells , Wolfgang Wurst and Achim Gossler
/ B2 ~% R: Y5 g0 P; A7. Classical genetics and gene targeting , Scott Bultman and Terry Magnuson 2 J+ y$ c/ a2 a
List of suppliers " N- a# E* Q5 c3 X/ f: J+ o
Index
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发表于 2010-7-1 12:43
