isl1心脏祖细胞贯穿于整个生命过程：从胚胎期至成年期

zhangjinxy

Islet-1 Cells Are Cardiac Progenitors Present During the Entire Lifespan: From the Embryonic Stage to Adulthood" U/ i; o' Z5 I2 E
Stem Cells Dev. 2010 Aug 28.
+ K1 U) X0 A% ?" i6 P5 }0 {         The aim of this study was to longitudinally characterize the distribution of cells actively expressing the progenitor transcription factor islet-1 (Isl1(+)) during the embryonic life, the postnatal period, and adulthood. In this study, we have used direct immunohistochemical staining toward the protein Isl1 in a longitudinal rat model. Cells actively expressing Isl1 were traced in embryos from gestational day (GD) 11 until adulthood. In early cardiac development (GD 11), the Isl1(+) progenitors were located in a greater abundance in the paracardiac regions, areas suggested to be the second heart field. To a lesser extent, Isl1(+) cells were present within the bulbotruncal region and the truncus arteriosus. During the following days until GD 15, the Isl1(+) cells were mainly observed at the proximal outflow tract (OFT) and at the inflow area of the right atrium. No Isl1(+) cells were detected in the left ventricle. Compared with GD 11, more Isl1(+) cells seemed to co-express cardiomyocyte markers and a minority of the Isl1(+) cells was undifferentiated. Unexpectedly, only few undifferentiated Isl1(+) cells were Ki67(+) while a lot of TnT(+) cardiomyocytes were proliferating in the ventricles. After birth, immature Isl1(+) cells were still present in the OFT where they resided until adulthood. Our data suggest that during embryogenesis, Isl1(+) cells migrate from extracardiac regions into the proximal part of the heart, proliferating and giving rise to cardioblasts. Unexpectedly, only a minority of the Isl1(+) cells while a majority of ventricular cardiomyocytes were proliferating. The Isl1(+) cell pool persists into adulthood, which might open up new strategies to repair damaged myocardium.

tonyp0720

好好研究一下，一直注意GATA4，NKX2.5 TBX5，这个都没注意，不知道跟心脏疾病有没有关系。

xmubely

回复 tonyp0720 的帖子
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isl1是SHF分化、发育过程中的一个关键TF，它的缺失会导致整个shf缺失，是致死性的。不过也有研究显示shf的SNP与先天性心脏病有关系。具体哪些心脏病，这个报道的好像还不多~

tonyp0720

个人觉得ISL1比较上游，做它的功能方面还是有点难度，现在大部分还是做几个具体的下游转录因子，也好出结果嘛 呵呵