The Myc Connection: ES Cells and Cancer

饶冠华

Myc Network Accounts for Similarities between Embryonic Stem and Cancer Cell Transcription Programs
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Jonghwan Kim, Andrew J. Woo, Jianlin Chu, Jonathan W. Snow, Yuko Fujiwara, Chul Geun Kim, Alan B. Cantor, Stuart H. OrkinSee AffiliationsHint: Rollover Authors and Affiliations Department of Pediatric Oncology, Children's Hospital and Dana Farber Cancer Institute, Boston, MA 02115, USA9 m! T5 `, l* h8 F- p6 Y

0 \5 A8 {, x& _; x0 MMyc associates with NuA4 complex, a critical regulator in ES cell identity ! N- G3 p0 O# M$ C7 Z
Three regulatory modules (Core, PrC, and Myc) are defined in ES cells
; D+ A3 H" D: N$ x1 LCore, PrC, and Myc modules are functionally separable : z$ g+ e3 [* M% g/ t0 M
Myc module accounts for the similarity of ES cell and cancer signatures4 L  j/ ]. D* F5 c4 o8 T
Summary
" b0 f6 B1 ~7 e" Gc-Myc (Myc) is an important transcriptional regulator in embryonic stem (ES) cells, somatic cell reprogramming, and cancer. Here, we identify a 2 values range from 0.96 to 0.99 for X. laevis and 0.94 to 0.98 for X. tropicalis. Error bars represent standard deviation (SD).
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& o" q, v1 Q: t* x0 @& U0 D+ B(C) X. laevis and X. tropicalis extracts were mixed as indicated, and nuclear size was measured at 90 min. One representative experiment of three is shown, and error bars represent SD.6 W' [! j# T* N; N6 w
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(D) Nuclei were assembled using the indicated source of extract and sperm, and nuclear size was measured at 90 min. One representative experiment of three is shown, and error bars represent SD.
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% ]$ ]! A8 x- ?, V: Q(E) GFP-NLS was added to nuclei at 30 min, and images were acquired live at 30 s intervals with the same exposure time. Nuclear GFP-NLS fluorescence intensity per unit area was measured at each time point, averaged for five nuclei from each extract, and normalized to 1.0 (arbitrary units). Error bars represent SD.

yourwish140

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cypress

有supplemental information提供吗？

ttyjimmy

好东西 谢啦