求文献一篇 Generation of human-induced

zhiguo1985

求文献一篇 Generation of human-induced pluripotent stem cells in the absence of exogenous Sox2% S# k3 f* X; Y9 X% n' \$ A- b% g
万分感谢！

hstudent

okok

zhiguo1985

再次谢谢回复 2# hstudent

zhiguo1985

您上传的是nature protool的文章
0 S2 r$ w6 j3 M5 Q# p: a# r: c我需要2009 《stem cell》的一篇文章，您有的话麻烦您上传一下，谢谢。- G& N* i: c4 v, ?6 J& x2 s! G6 u
NCBI: http://www.ncbi.nlm.nih.gov/pubmed/198390557 ~4 v3 X8 a( V4 k' Q

9 ~% V" Y. H6 }! |) N+ HStem Cells. 2009 Dec;27(12):2992-3000." j: x' h* E8 Q3 F9 C
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Generation of human-induced pluripotent stem cells in the absence of exogenous Sox2.
7 o; l9 R5 Z6 S, E. r0 V; ULi W, Zhou H, Abujarour R, Zhu S, Young Joo J, Lin T, Hao E, Schöler HR, Hayek A, Ding S.
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3 ]$ B6 u9 }, O* w- oDepartment of Chemistry, The Scripps Research Institute, La Jolla, California 92037, USA.* q* B5 Q5 k- c8 N5 p1 U) Y9 ~$ j

6 z& F# }' `1 l9 E* f0 tAbstract) K6 g6 A, @% v$ z/ I& o/ _0 L
Induced pluripotent stem cell technology has attracted enormous interest for potential application in regenerative medicine. Here, we report that a specific glycogen synthase kinase 3 (GSK-3) inhibitor, CHIR99021, can induce the reprogramming of mouse embryonic fibroblasts transduced by only two factors, Oct4 and Klf4. When combined with Parnate (also named tranylcypromine), an inhibitor of lysine-specific demethylase 1, CHIR99021 can cause the reprogramming of human primary keratinocyte transduced with the two factors, Oct4 and Klf4. To our knowledge, this is the first time that human iPS cells have been generated from somatic cells without exogenous Sox2 expression. Our studies suggest that the GSK-3 inhibitor might have a general application to replace transcription factors in both mouse and human reprogramming.
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PMID: 19839055 [PubMed - indexed for MEDLINE]

linlicau

回复 4# zhiguo1985 # C# P$ o' [* K& C( o9 t% e

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zhiguo1985

谢谢

ayeqiu

刚好是我要找的资料，O(∩_∩)O谢谢了！~