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本帖最后由 细胞海洋 于 2010-11-7 17:45 编辑
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Cytoskeleton Methods and Protocols
5 H# f# J" a9 {) Hvon Ray H. Gavin' F; ]6 v6 V& o7 x7 F
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Kategorie: Molekularbiologie3 f1 f! m, E$ T# z. i' N
ISBN: 0896037711
# {( p! M" i9 {/ A9 `6 v QFrom Book News, Inc. A collection of methods that utilize biochemistry, immunology, genetics, microscopy, and image analysis for investigating cytoskeleton structure and function. The protocols utilize several model systems in a variety of organisms, including Saccharomyces, Micrasterias, Tetrahymena, Drosophila, Spisula, and Xenopus. The manual consists of 22 chapters with such topics as: an inverse PCR strategy to clone large contiguous genomic DNA fragments, computer-assisted systems for the analysis of amoeboid cell mobility, an actin-specific reagent that promotes actin polymerization, the use of green fluorescent protein, and detergent-extracted models for the study of cilia or flagella.Book News, Inc.®, Portland, OR
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Synopsis This volume provides techniques for investigating the integration of cytoskeleton structure and function. An international panel of experienced investigators present detailed protocols that combine biochemistry, immunology, genetics, microscopy, and image analysis in integrative approaches for investigations of cytoskeleton components. Each protocol is provided with step-by-step instructions and informative notes that enable both novice and experienced investigators to successfully use the...
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