关于N2和B27.咨询大家！

随风的天使

发现一些文献中在培养胚胎干细胞时采用了N2和B27，请教大家。介绍一下这两种物质的作用？在培养时怎样使用？那个公司的比较好？谢谢大家。

jennyshy

invitrogen公司的2 e+ C) |' P# @. E+ _6 N5 }7 ?
关于介绍他们的作用，网站上有你自己找找3 n* G% g) O' @& `5 c+ Y4 T/ }
或者看产品说明

BIOLOGY

N2主要含5中成分，两种蛋白，即human transferrin, insulin recombinant full chain;
  d3 q4 I2 }3 m1 `$ F) \4 O还有其他三种成分：progesterone, putrescine, selenite
4 O( B: n' A* n# f" G; z1 c/ wN2 Supplement is a serum-free supplement for the growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype.  N2 supplement is a chemically defined, 100X concentrate of Bottenstein's N2 formulation.  This supplement is recommended for the growth and expression of neuroblastomas and for the survival and expression of post-mitotic neurons in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS).  It can be used as a substitute for the N1 Bottenstein formulation.  N2 medium appears to be selective for neuronal cell lines and does not support the growth of nonneuronal cell lines

BIOLOGY

B-27® Supplement is an optimized serum-free supplement used to support the low or high density growth and short or long-term viability of hippocampal and other CNS neurons. B-27® B27 Supplement is provided as a 50X liquid and is intended to be used   Morewith Neurobasal® Medium or Neurobasal®-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.
" x+ t5 |6 Y  _: g6 p$ P. V详细信息楼主可以去Invitrogen网站上查找% o7 o% _" l" k9 n; i- z
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随风的天使

回复 jennyshy 的帖子
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非常感谢。

随风的天使

回复 BIOLOGY 的帖子+ l. ~# C* o$ ?9 J
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非常感谢，有了一些了解了，这个是否可以用于胚胎干细胞的培养呀？

随风的天使

回复 BIOLOGY 的帖子+ Q) S, L- @  W. ]' V4 Q

8 T- U, ~) S( G7 |& u) |% H& n谢谢你，我去查查看。

BIOLOGY

回复 随风的天使 的帖子
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8 Z* K' }- [- P. i5 u1 C2 ]可以用于胚胎干细胞的培养，尤其是N2用的比较多一些，具体也要看你要养什么细胞

jennyshy

回复 BIOLOGY 的帖子% H& {; ]& r' y( n+ Q
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ESC要用invitrogen的KO-SR，分化时可以用B27

随风的天使

回复 jennyshy 的帖子; k( x& M0 v/ Y7 T

1 h$ P2 N. q6 T& ]5 [+ [4 r# F非常感谢