In Vitro Differentiation of ES Cells into Cardiac Muscle胚胎干细胞体外分化成心肌

肖爱华

In Vitro Differentiation of ES Cells into Cardiac Muscle胚胎干细胞体外分化成心肌
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Day -1: Pass ES cells at normal density on gelatinized plate to free the culture of contamination fibroblast cells.9 ]* ]$ Z0 A7 B% _% A- z

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Day 1: Trypsinized the cells as for normal passaging until the colonies lift off. Try to keep the loosely connected clumps of cells together by gentle handling. Then directly plate the cells 1:3 into bacterial grade Petri dishes in LIF free ES cell medium.7 d) i$ B7 s+ _/ b0 ?* k

. {; ]3 M7 }# Z8 ?5 q0 W, NDay 3: Aspirate the medium carefully. Avoid sucking up too many of the aggregates. Then add new medium.9 K% R, o! g! K/ Y: O4 |. d
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Day 5: Aspirate as in Day 3 and replace the medium.
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# R. q8 i4 w5 J' g. a8 F' G+ `/ cDay 7: Plate the cells into 24 weel tissue culture grade plate+ y# h; W0 O! X2 ~
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Day 9: Change half of the medium and observe beating, u7 P2 y+ E; y9 G# \$ `. V

' h) o# M0 [5 i, l! e& hDay 11: Change half of the medium and observe beating