Picking ES Cell Colonies and Transferring Them to 24-Well Culture Dishes

肖爱华

Picking ES Cell Colonies and Transferring Them to 24-Well Culture Dishes From the Laboratory of Dr. Allan Bradley & r! }0 R" B" p' _- g
Baylor College of Medicine, Houston, Texas
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Picking ES Cell Colonies and
8 f4 r2 U* _' UTransferring Them to 24-Well Culture Dishes
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        1.        Spot 15 - 25 ul of trypsin in a 24-well array onto the lid
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        2.        After flooding the plate with PBS, pick the colony from $ Y7 S$ @# a# P( A- j( `- t
the plate using a Pipetman set at 2 ul, and transfer it into the trypsin.  * ?6 ^: A0 e$ m+ m5 z4 y" }9 Z
Leave in trypsin for 10-15 minutes.- }% h/ m9 r3 [" C9 L5 l' S

' U% r  I. K& R/ O        3.        Add 20 ul of media from the refed feeder plate to the ( n4 ]* P, F. j) |* U
trypsin spot.  Pipet up and down to break up the colony.' e( m' A, \" |3 X2 b( R1 k! r

3 C; j) I% |4 ]$ {: L' U        4.        Transfer the ES cell suspension (approximately 37 ul)
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        5.        Alternatively, 50% of the cells may be lysed directly 8 W" }* Q6 G# j; p
for PCR at this point.  Add 15 ul of the cell suspension directly
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From the Laboratory of Dr. Allan Bradley                     
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可怜的孩子

谢谢啊

llingzu

kankan

sacia

学习了，谢谢楼主啦