利用新型复合支架材料及牙髓干细胞进行牙齿重建

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原文来源自TISSUE ENGINEERING AND REGENERATIVE MEDICINE    由干细胞之家新闻小组成员hxf_86翻译（转帖请注明）/ m% d* E+ d, U3 K( v2 [
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利用新型复合支架材料及牙髓干细胞进行牙齿重建2 E: k5 m7 v  `

3 c' b0 b  U) o8 d* U6 p摘要：在这个研究中，主要是研究复合支架材料的特点及生物相容性，而这种支架材料可以应用在牙髓干细胞进行牙齿重建中。这种复合材料由poly-D,L-lactic acid (P(D,L)LA)及从烧结的牙齿中得到的生物磁粉材料组成。高渗透性的复合生物磁粉支架提高了生物相容性，并且有相互连接的孔，可以给细胞的粘附与增值提供足够的支持。同时，我们对比通过以下两种方法制造的复合支架材料的特点及生物相容性：溶剂浇铸/颗粒浸出法及气成形方法。我们利用从拔出的牙齿中得到的牙齿磷灰石准备这个生物陶瓷支架，并通过两种方法去评估它的生物相容性，以期找到牙齿重建合适的支架材料。通过细胞毒性测试、细胞粘附测试及组织学的研究，发现人牙髓干细胞接种到复合支架后很容易粘附并且增值良好。本实验结果可以证明，复合骨组织支架材料是一种用于牙髓干细胞进行牙齿重建时合适的支架材料。
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! {, j" D7 `' u3 f% n, `% n$ r# N- ONovel Composite Scaffolds for Tooth Regeneration using Human Dental Pulp Stem Cells
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来源出版物: TISSUE ENGINEERING AND REGENERATIVE MEDICINE    卷: 7    期: 5    页: 473-480    出版年: DEC 2010  
' i! l( Q6 b5 E. @) d$ J' Q) Z3 j& o摘要: In this study, the properties and biocompatibilities of composite scaffolds were investigated to develop an appropriate scaffold for dental tissue regeneration using human dental pulp stem cells. Composite scaffolds consisted of poly-D,L-lactic acid (P(D,L)LA) and bioceramic materials of tooth powders fabricated using sintered teeth. Highly porous composite bioceramic scaffolds promoted biocompatibility and had the interconnected pores that could adequately support cell adhesion and proliferation. Also, we investigated the properties and biocompatibilities of composite scaffolds prepared by a solvent casting/particulate leaching method and a gas forming method. We prepared the composite ceramic scaffold using toothapatites obtained from extracted teeth, and assessed the biocompatibility of the composite scaffolds by the two methods in order to find out the most appropriate scaffolds for tooth regeneration. The human dental pulp stem cells seeded on the composite scaffolds were easily attached and well proliferated as confirmed by a cytotoxicity test, cell adhesion assessment and histological study. Through experimental results, composite bone scaffolds were able to be proven as appropriate scaffolds for dental tissue regeneration using human dental pulp stem cells.. u! M* N& v4 T# v! J

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