干细胞小生境stem cell niche有哪些些调节方式研究？

swxxx

种系干细胞小生境调节的方式有哪些？空间体积大小，与与周围细胞协同发育。还有那些分子机制？具体那些研究方法?

varing

在小鼠中spermatogonial stem cell的niche主要认为是sertoli cell、myoid cell 以及曲精小管之间的间质空间(intersitium space) 细胞如Leydig cell 以及间质中的血管、淋巴管。SSC主要分部在邻近intersitium的区域，可见intersitium的重要性（调控SSC的干性和分化）sertoli cell能分泌多种因子如神经胶质细胞源性神经营养因子（GDNF），sertoli cell的分泌又受myoid 、leydig cell、血管中激素的调控。

yusheen

Stem cell niche is a phrase loosely used in the scientific community to describe the microenvironment in which stem cells are found, which interacts with stem cells to regulate stem cell fate. The word 'niche' can be in reference to the in vivo or in vitro stem cell microenvironment. During embryonic development, various niche factors act on embryonic stem cells to alter gene expression, and induce their proliferation or differentiation for the development of the fetus. Within the human body, stem cell niches maintain adult stem cells in a quiescent state, but after tissue injury, the surrounding micro-environment actively signals to stem cells to either promote self renewal or differentiation to form new tissues. Several factors are important to regulate stem cell characteristics within the niche: cell-cell interactions between stem cells, as well as interactions between stem cells and neighbouring differentiated cells, interactions between stem cells and adhesion molecules, extracellular matrix components, the oxygen tension, growth factors, cytokines, and physiochemical nature of the environment including the pH, ionic strength (e.g. Ca2+ concentration) and metabolites, like ATP, are also important. The stem cells and niche may induce each other during development and reciprocally signal to maintain each other during adulthood.
) K2 D  `* F6 hScientists are studying the various components of the niche and trying to replicate the in vivo niche conditions in vitro. This is because for regenerative therapies, cell proliferation and differentiation must be controlled in flasks or plates, so that sufficient quantity of the proper cell type are produced prior to being introduced back into the patient for therapy.
. O7 w& C# \+ X/ l9 HHuman embryonic stem cells are often grown in fibroblastic growth factor-2 containing, fetal bovine serum supplemented media. They are grown on a feeder layer of cells, which is believed to be supportive in maintaining the pluripotent characteristics of embryonic stem cells. However, even these conditions may not truly mimic in vivo niche conditions.4 e9 B- L& \: h0 v' H, x7 Y7 X
Adult stem cells remain in an undifferentiated state throughout adult life. However, when they are cultured in vitro, they often undergo an 'aging' process in which their morphology is changed and their proliferative capacity is decreased. It is believed that correct culturing conditions of adult stem cells needs to be improved so that adult stem cells can maintain their stemness over time.  f2 i' Y6 q9 F8 ^9 d3 Q0 {/ H7 B
A Nature Insight review defines niche as follows:
: v7 s1 l. s0 Q5 ^"Stem-cell populations are established in 'niches' — specific anatomic locations that regulate how they participate in tissue generation, maintenance and repair. The niche saves stem cells from depletion, while protecting the host from over-exuberant stem-cell proliferation. It constitutes a basic unit of tissue physiology, integrating signals that mediate the balanced response of stem cells to the needs of organisms. Yet the niche may also induce pathologies by imposing aberrant function on stem cells or other targets. The interplay between stem cells and their niche creates the dynamic system necessary for sustaining tissues, and for the ultimate design of stem-cell therapeutics...The simple location of stem cells is not sufficient to define a niche. The niche must have both anatomic and functional dimensions"
+ W$ m, E3 Z- e* L* M1 E—David T. Scadden, The stem-cell niche as an entity of action, Nature, 441 (7097), 1075-1079 (29 June 2006)0 @7 c9 Y* c  l% N
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