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回复 tpwang 的帖子! ?2 h2 W- R! B- |8 ?) B6 I* f9 N* k
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一篇从分化结果来比较iPS和ESC的新文章/ } ?% r+ ]! I# |! C
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Epigenetic and Phenotypic Profile of Fibroblasts Derived from Induced Pluripotent Stem Cells3 y* w* ^& i9 G8 a1 f
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iPS和ESC的一些差异是否必然会延续到分化过程以及分化结果,并在体内功能上会有差异,还没有定论。是否这些差异会在分化过程中消除或减弱,也是一种理论上的可能性。
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Human induced pluripotent stem (hiPS) cells offer a novel source of patient-specific cells for regenerative medicine. However, the biological potential of iPS-derived cells and their similarities to cells differentiated from human embryonic stem (hES) cells remain unclear. We derived fibroblast-like cells from two hiPS cell lines and show that their phenotypic properties and patterns of DNA methylation were similar to that of mature fibroblasts and to fibroblasts derived from hES cells. iPS-derived fibroblasts (iPDK) and their hES-derived counterparts (EDK) showed similar cell morphology throughout differentiation, and patterns of gene expression and cell surface markers were characteristic of mature fibroblasts. Arraybased methylation analysis was performed for EDK, iPDK and their parental hES and iPS cell lines, and hierarchical clustering revealed that EDK and iPDK had closely-related methylation profiles. DNA methylation analysis of promoter regions associated with extracellular matrix (ECM)-production (COL1A1) by iPS- and hESC-derived fibroblasts and fibroblast lineage commitment (PDGFRb), revealed promoter demethylation linked to their expression, and patterns of transcription and1 W4 [$ L5 [$ I# t/ H; L
methylation of genes related to the functional properties of mature stromal cells were seen in both hiPS- and hES-derived fibroblasts. iPDK cells also showed functional properties analogous to those of hES-derived and mature fibroblasts, as seen by their capacity to direct the morphogenesis of engineered human skin equivalents. Characterization of the functional behavior of ES- and iPS-derived fibroblasts in engineered 3D tissues demonstrates the utility of this tissue platform to predict the capacity of iPS-derived cells before their therapeutic application.
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