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xiaomao_zhoubo

【题名】：Selective decline of the metabolic competence of oversized synaptic mitochondria in the old monkey cerebellum
! }1 c$ q6 b. _; B% |3 o- n8 a1 I( X【作者】：Bertoni-Freddari, C.   Balietti, M.    Giorgetti, B.! {7 A1 z( M( s  z% V
【杂志全称或缩写】：Rejuvenation Res
# u  T6 W- R8 N+ o! v5 M【年份，卷（期），起止页码】：2008;11(2); 387-91
- N+ i0 z/ y1 w% ^4 ^& u【PMID】：183280031 Q. [- C% _# i! ^! S5 ]
【全文链接】：http://www.ncbi.nlm.nih.gov/entr ... ;list_uids=18328003* J) @, R6 c& g% A1 k

inankai

Rejuvenation Res. 2008 Apr;11(2):387-91.) ?  ~7 k8 j7 d+ D
Selective decline of the metabolic competence of oversized synaptic mitochondria in the old monkey cerebellum.
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9 B+ b3 b" N1 f& `: yBertoni-Freddari C, Balietti M, Giorgetti B, Grossi Y, Casoli T, Di Stefano G, Perretta G, Fattoretti P.
% {( y7 R6 s4 A0 S9 ~# l/ Y9 E7 h! L4 Q
Neurobiology of Aging Laboratory, INRCA Research Department, Ancona, Italy. c.bertoni@inrca.it
* l9 {$ W5 O! D- u0 x/ KAbstract8 t: {* @! b; s9 K, ]& j( B* O9 K( C

& k6 a; d/ j1 k: pThe morphofunctional features of synaptic mitochondria, positive to the activity of cytochrome oxidase (COX), were investigated in the cerebellar cortex of adult and old monkeys (Macaca fascicularis) to assess the potential age-related changes in the energy metabolism occurring at the neuronal synaptic compartment. The following mitochondrial ultrastructural parameters-numeric density (Nv), volume density (Vv), average volume (V), and average length (Fmax)-were measured by computer-assisted morphometric methods. The ratio (R) area of the COX cytochemical precipitate/area of the mitochondrion was semi-automatically calculated and considered as an estimation of the mitochondrial metabolic competence (MMC), that is, the capacity of single organelles to provide adequate amounts of adenosinetriphosphate. No age-related significant differences were found in any of the ultrastructural parameters taken into account, whereas a significant decrease of R was observed in old animals. In these animals, the quartile distribution of the COX-positive organelles, according to their respective cross-sectional area, showed no significant difference of R when comparing small (I quartile), medium-sized (II quartile), and large (III quartile) mitochondria, while a significant decrease of R was evident in oversized mitochondria (IV quartile). Although our data document an age-related preservation of the morphological features of COX-positive mitochondria in the monkey cerebellum, the significant decrease of R in old animals needs to be considered from the functional standpoint. Since COX is the terminal enzyme of the mitochondrial respiratory chain, the estimation of its activity is regarded as a reliable MMC index; thus our findings, by matching preferential cytochemistry and morphometry, support the hypothesis that the specific functional impairment of enlarged synaptic mitochondria may seriously affect information processing and cell-to-cell communication at synaptic junctional areas with aging.

xiaomao_zhoubo

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