hES和iPS细胞的基因图谱比较（大略）

hyde

Reference Maps of Human ES and iPS Cell Variation Enable High-Throughput Characterization of Pluripotent Cell Lines, Q, d" L, R3 q7 J8 }

( k: R' ~& z- w, @6 w1 I7 f原文来自于http://www.sciencedirect.com/sci ... 16&searchtype=a8 l2 C  j9 c# n, {. Q
有干细胞之家Hyde翻译，转帖请注明。: K4 Y! i2 m* E/ H% r& K
译文：人多能干细胞具有的发育潜能说明了这些细胞能够产生用于生物医药研究的疾病相关细胞类型。但是有报道表明这些多能细胞系之间存在着巨大的差异，这些差异影响了它们的使用和临床安全性。在翻译研究（？）中放心使用ESC和iPSC之前必须要对这些细胞系间的特异性差异做更深入地了解。为了达到这一目的，我们建立了DNA甲基化、HES基因表达、hiPS基因表达的全基因组参考图谱，其中hES是以前建立的20株细胞。我们已经检测了这些细胞的体外分化偏向性。这些资源让我们能够评估ES和iPS在表观遗传学、转录上的相似性，以及各细胞系的分化效率。结合这些分析归纳出一张“打分卡”用于快速而综合地评价多能细胞系的特性。4 d. Y# Z( `; d
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The developmental potential of human pluripotent stem cells suggests that they can produce disease-relevant cell types for biomedical research. However, substantial variation has been reported among pluripotent cell lines, which could affect their utility and clinical safety. Such cell-line-specific differences must be better understood before one can confidently use embryonic stem (ES) or induced pluripotent stem (iPS) cells in translational research. Toward this goal we have established genome-wide reference maps of DNA methylation and gene expression for 20 previously derived human ES lines and 12 human iPS cell lines, and we have measured the in vitro differentiation propensity of these cell lines.This resource enabled us to assess the epigenetic and transcriptional similarity of ES and iPS cells and to predict the differentiation efficiency of individual cell lines. The combination of assays yields a scorecard for quick and comprehensive characterization of pluripotent cell lines.

aulenuyah

楼主能把原文放在附件里吗，谢谢了

hyde

hyde

回复 aulenuyah 的帖子5 i3 m& [; |4 j& l

3 {- K% O5 a) |忘了，刚做实验去了，谢谢提醒，

tpwang

回复 hyde 的帖子, l4 l2 V: |$ ^/ t2 T! X& `
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几点小提示：
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* R( Y) l. K+ V8 \6 n  ?# gcould用在这里是一种可能语气，意思是这些差异“有可能会影响它们的临床应用价值和安全性”。一个典型句型的例句："It could be years before" we can harvest the benefits of stem cells.
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translational research=（临床）转化研究。介于basic research和applied research之间的一个关键环节。Quote：Generally speaking, the primary goal of “translational” research is to integrate advancements in molecular biology with clinical trials, taking research from the “bench-to-bedside”.  本站下载量很高的所谓ISSCR“干细胞临床应用手册”其实是“干细胞临床转化指导意见”（Guidelines for the Clinical Translation of Stem Cells）。它只是用于指导研究者如何进行干细胞临床转化研究，而不是临床应用的标准和规则。ISSCR作为一个学术协会是无权制定规则的，而且也不是一个临床专业协会。它有一个相应的The ISSCR Task Force for the Clinical Translation of Stem Cells。临床转化研究是近些年来很流行的一个概念和领域。
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: t9 n  Z1 d& I% E" _+ y5 Mdifferentiation propensity翻译为分化“趋向性”也可。
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+ w' `  e( H% |comprehensive这里翻译为“全面”更准确些。6 s+ d3 [( A. U  R/ U

3 ~( m$ Q: l8 v) [9 A供参考。
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hyde

回复 tpwang 的帖子
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9 l. c* K3 t" N  i) j6 k2 P9 p有长知识了，非常谢谢
9 {- r; g3 P( _; j& I尤其是“translational research”，第一次见到，所以没处理好。7 ~) ?3 d/ p4 X. d

jane1lee

谢谢~~学习了

farmer

标记。